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| Title | The respiratory syncytial virus nucleoprotein-RNA complex forms a left-handed helical nucleocapsid. |
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| Journal, issue, pages | J Gen Virol, Vol. 94, Issue Pt 8, Page 1734-1738, Year 2013 |
| Publish date | May 15, 2013 |
 Authors | Saskia E Bakker / Stéphane Duquerroy / Marie Galloux / Colin Loney / Edward Conner / Jean-François Eléouët / Félix A Rey / David Bhella /   |
| PubMed Abstract | Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical ...Respiratory syncytial virus (RSV) is an important human pathogen. Its nucleocapsid (NC), which comprises the negative sense RNA viral genome coated by the viral nucleoprotein N, is a critical assembly that serves as template for both mRNA synthesis and genome replication. We have previously described the X-ray structure of an NC-like structure: a decameric ring formed of N-RNA that mimics one turn of the helical NC. In the absence of experimental data we had hypothesized that the NC helix would be right-handed, as the N-N contacts in the ring appeared to more easily adapt to that conformation. We now unambiguously show that the RSV NC is a left-handed helix. We further show that the contacts in the ring can be distorted to maintain key N-N-protein interactions in a left-handed helix, and discuss the implications of the resulting atomic model of the helical NC for viral replication and transcription. |
 External links | J Gen Virol / PubMed:23677789 / PubMed Central |
| Methods | EM (subtomogram averaging) / EM (tomography) |
| Structure data | EMDB-2369, PDB-4bkk:  EMDB-2370: |
| Source |
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 Keywords |  VIRAL PROTEIN/RNA / VIRAL PROTEIN-RNA COMPLEX |
