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| Title | Dynamical features of the Plasmodium falciparum ribosome during translation. |
|---|---|
| Journal, issue, pages | Nucleic Acids Res, Vol. 43, Issue 21, Page 10515-10524, Year 2015 |
| Publish date | Dec 2, 2015 |
 Authors | Ming Sun / Wen Li / Karin Blomqvist / Sanchaita Das / Yaser Hashem / Jeffrey D Dvorin / Joachim Frank /    |
| PubMed Abstract | Plasmodium falciparum, the mosquito-transmitted Apicomplexan parasite, causes the most severe form of human malaria. In the asexual blood-stage, the parasite resides within erythrocytes where it ...Plasmodium falciparum, the mosquito-transmitted Apicomplexan parasite, causes the most severe form of human malaria. In the asexual blood-stage, the parasite resides within erythrocytes where it proliferates, multiplies and finally spreads to new erythrocytes. Development of drugs targeting the ribosome, the site of protein synthesis, requires specific knowledge of its structure and work cycle, and, critically, the ways they differ from those in the human host. Here, we present five cryo-electron microscopy (cryo-EM) reconstructions of ribosomes purified from P. falciparum blood-stage schizonts at sub-nanometer resolution. Atomic models were built from these density maps by flexible fitting. Significantly, our study has taken advantage of new capabilities of cryo-EM, in visualizing several structures co-existing in the sample at once, at a resolution sufficient for building atomic models. We have discovered structural and dynamic features that differentiate the ribosomes of P. falciparum from those of mammalian system. Prompted by the absence of RACK1 on the ribosome in our and an earlier study we confirmed that RACK1 does not specifically co-purify with the 80S fraction in schizonts. More extensive studies, using cryo-EM methodology, of translation in the parasite will provide structural knowledge that may lead to development of novel anti-malarials. |
 External links | Nucleic Acids Res / PubMed:26432834 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 4.7 - 6.7 Å |
| Structure data | EMDB-6452: Cryo electron-microscopy reconstruction of the P. falciparum 80S ribosome bound with A/P and P/E tRNAs |
| Source |
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 Keywords |  RIBOSOME |
