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TitleStructure of the TRPA1 ion channel suggests regulatory mechanisms.
Journal, issue, pagesNature, Vol. 520, Issue 7548, Page 511-517, Year 2015
Publish dateApr 23, 2015
AuthorsCandice E Paulsen / Jean-Paul Armache / Yuan Gao / Yifan Cheng / David Julius /
PubMed AbstractThe TRPA1 ion channel (also known as the wasabi receptor) is a detector of noxious chemical agents encountered in our environment or produced endogenously during tissue injury or drug metabolism. ...The TRPA1 ion channel (also known as the wasabi receptor) is a detector of noxious chemical agents encountered in our environment or produced endogenously during tissue injury or drug metabolism. These include a broad class of electrophiles that activate the channel through covalent protein modification. TRPA1 antagonists hold potential for treating neurogenic inflammatory conditions provoked or exacerbated by irritant exposure. Despite compelling reasons to understand TRPA1 function, structural mechanisms underlying channel regulation remain obscure. Here we use single-particle electron cryo- microscopy to determine the structure of full-length human TRPA1 to ∼4 Å resolution in the presence of pharmacophores, including a potent antagonist. Several unexpected features are revealed, including an extensive coiled-coil assembly domain stabilized by polyphosphate co-factors and a highly integrated nexus that converges on an unpredicted transient receptor potential (TRP)-like allosteric domain. These findings provide new insights into the mechanisms of TRPA1 regulation, and establish a blueprint for structure-based design of analgesic and anti-inflammatory agents.
External linksNature / PubMed:25855297 / PubMed Central
MethodsEM (single particle)
Resolution3.92 - 4.67 Å
Structure data

6267

3j9p

EMDB-6267: Human TRPA1 ion channel with agonist AITC
PDB-3j9p: Structure of the TRPA1 ion channel determined by electron cryo-microscopy
Method: EM (single particle) / Resolution: 4.24 Å

EMDB-6268:
Human TRPA1 ion channel with double antagonist HC030031/A967079
Method: EM (single particle) / Resolution: 3.92 Å

EMDB-6269:
Human TRPA1 ion channel with antagonist HC030031
Method: EM (single particle) / Resolution: 4.67 Å

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsTRANSPORT PROTEIN / TRPA1 / TRP / transient / potential / receptor / ion channel / membrane protein

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