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TitleA flipped ion pair at the dynein-microtubule interface is critical for dynein motility and ATPase activation.
Journal, issue, pagesJ Cell Biol, Vol. 208, Issue 2, Page 211-222, Year 2015
Publish dateJan 19, 2015
AuthorsSeiichi Uchimura / Takashi Fujii / Hiroko Takazaki / Rie Ayukawa / Yosuke Nishikawa / Itsushi Minoura / You Hachikubo / Genji Kurisu / Kazuo Sutoh / Takahide Kon / Keiichi Namba / Etsuko Muto /
PubMed AbstractDynein is a motor protein that moves on microtubules (MTs) using the energy of adenosine triphosphate (ATP) hydrolysis. To understand its motility mechanism, it is crucial to know how the signal of ...Dynein is a motor protein that moves on microtubules (MTs) using the energy of adenosine triphosphate (ATP) hydrolysis. To understand its motility mechanism, it is crucial to know how the signal of MT binding is transmitted to the ATPase domain to enhance ATP hydrolysis. However, the molecular basis of signal transmission at the dynein-MT interface remains unclear. Scanning mutagenesis of tubulin identified two residues in α-tubulin, R403 and E416, that are critical for ATPase activation and directional movement of dynein. Electron cryomicroscopy and biochemical analyses revealed that these residues form salt bridges with the residues in the dynein MT-binding domain (MTBD) that work in concert to induce registry change in the stalk coiled coil and activate the ATPase. The R403-E3390 salt bridge functions as a switch for this mechanism because of its reversed charge relative to other residues at the interface. This study unveils the structural basis for coupling between MT binding and ATPase activation and implicates the MTBD in the control of directional movement.
External linksJ Cell Biol / PubMed:25583999 / PubMed Central
MethodsEM (helical sym.)
Resolution8.2 Å
Structure data

5931

3j6p

EMDB-5931: Dictyostelium dynein microtubule binding domain bound to a 15-protofilament microtubule
PDB-3j6p: Pseudo-atomic model of dynein microtubule binding domain-tubulin complex based on a cryoEM map
Method: EM (helical sym.) / Resolution: 8.2 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • dictyostelium discoideum (eukaryote)
  • Caenorhabditis elegans (invertebrata)
  • sus scrofa (pig)
KeywordsMOTOR PROTEIN/STRUCTURAL PROTEIN / Motor Protein-Cytoskeleton Complex / MOTOR PROTEIN-STRUCTURAL PROTEIN complex

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