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Structure paper

TitleThe Ndc80 kinetochore complex forms oligomeric arrays along microtubules.
Journal, issue, pagesNature, Vol. 467, Issue 7317, Page 805-810, Year 2010
Publish dateOct 14, 2010
AuthorsGregory M Alushin / Vincent H Ramey / Sebastiano Pasqualato / David A Ball / Nikolaus Grigorieff / Andrea Musacchio / Eva Nogales /
PubMed AbstractThe Ndc80 complex is a key site of regulated kinetochore-microtubule attachment (a process required for cell division), but the molecular mechanism underlying its function remains unknown. Here we ...The Ndc80 complex is a key site of regulated kinetochore-microtubule attachment (a process required for cell division), but the molecular mechanism underlying its function remains unknown. Here we present a subnanometre-resolution cryo-electron microscopy reconstruction of the human Ndc80 complex bound to microtubules, sufficient for precise docking of crystal structures of the component proteins. We find that the Ndc80 complex binds the microtubule with a tubulin monomer repeat, recognizing α- and β-tubulin at both intra- and inter-tubulin dimer interfaces in a manner that is sensitive to tubulin conformation. Furthermore, Ndc80 complexes self-associate along protofilaments through interactions mediated by the amino-terminal tail of the NDC80 protein, which is the site of phospho-regulation by Aurora B kinase. The complex's mode of interaction with the microtubule and its oligomerization suggest a mechanism by which Aurora B could regulate the stability of load-bearing kinetochore-microtubule attachments.
External linksNature / PubMed:20944740 / PubMed Central
MethodsEM (helical sym.)
Resolution8.6 Å
Structure data

5223

3iz0

EMDB-5223, PDB-3iz0:
Human Ndc80 Bonsai Decorated Microtubule
Method: EM (helical sym.) / Resolution: 8.6 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • bos taurus (cattle)
  • homo sapiens (human)
KeywordsCELL CYCLE / Ndc80 / HEC1 / NUF2 / tubulin / kinetochore / mitosis / calponin homology domain / microtubule

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