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TitleThe cryo-EM structure of a translation initiation complex from Escherichia coli.
Journal, issue, pagesCell, Vol. 121, Issue 5, Page 703-712, Year 2005
Publish dateJun 3, 2005
AuthorsGregory S Allen / Andrey Zavialov / Richard Gursky / Måns Ehrenberg / Joachim Frank /
PubMed AbstractThe 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex ...The 70S ribosome and its complement of factors required for initiation of translation in E. coli were purified separately and reassembled in vitro with GDPNP, producing a stable initiation complex (IC) stalled after 70S assembly. We have obtained a cryo-EM reconstruction of the IC showing IF2*GDPNP at the intersubunit cleft of the 70S ribosome. IF2*GDPNP contacts the 30S and 50S subunits as well as fMet-tRNA(fMet). IF2 here adopts a conformation radically different from that seen in the recent crystal structure of IF2. The C-terminal domain of IF2 binds to the single-stranded portion of fMet-tRNA(fMet), thereby forcing the tRNA into a novel orientation at the P site. The GTP binding domain of IF2 binds to the GTPase-associated center of the 50S subunit in a manner similar to EF-G and EF-Tu. Additionally, we present evidence for the localization of IF1, IF3, one C-terminal domain of L7/L12, and the N-terminal domain of IF2 in the initiation complex.
External linksCell / PubMed:15935757
MethodsEM (single particle)
Resolution13.8 Å
Structure data

1248

1zo1

1zo3

EMDB-1248: The cryo-EM structure of a translation initiation complex from Escherichia coli.
PDB-1zo1: IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex
PDB-1zo3: The P-site and P/E-site tRNA structures fitted to P/I site codon.
Method: EM (single particle) / Resolution: 13.8 Å

1249

1zo1

1zo3

EMDB-1249: The cryo-EM structure of a translation initiation complex from Escherichia coli.
PDB-1zo1: IF2, IF1, and tRNA fitted to cryo-EM data OF E. COLI 70S initiation complex
PDB-1zo3: The P-site and P/E-site tRNA structures fitted to P/I site codon.
Method: EM (single particle) / Resolution: 13.8 Å

Source
  • escherichia coli (E. coli)
Keywordstranslation/RNA / E. COLI / RIBOSOME / INITIATION OF PROTEIN SYNTHESIS / INITIATION FACTOR / CRYO-ELETRON MICROSCOPY / translation-RNA COMPLEX / RNA

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