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TitleA new cryo-EM system for single particle analysis.
Journal, issue, pagesJ Struct Biol, Vol. 207, Issue 1, Page 40-48, Year 2019
Publish dateJul 1, 2019
AuthorsTasuku Hamaguchi / Saori Maki-Yonekura / Hisashi Naitow / Yoshinori Matsuura / Tetsuya Ishikawa / Koji Yonekura /
PubMed AbstractA new cryo-EM system has been investigated for single particle analysis of protein structures. The system provides parallel illumination of a highly-coherent 300 kV electron beam from a cold-field ...A new cryo-EM system has been investigated for single particle analysis of protein structures. The system provides parallel illumination of a highly-coherent 300 kV electron beam from a cold-field emission gun, and boosts image contrast with an in-column energy filter and a hole-free phase plate. It includes motorized cryo-sample loading and automated liquid-nitrogen filling for cooling multiple samples. In this study, we describe gun and electron beam characteristics, and demonstrate the suitability of this system for single particle reconstructions. The performance of the system is tested on two examples, a spherical virus and apoferritin. GUI programs have also been developed to control and monitor the system for correct illumination, imaging with less ellipticity and steady magnification, and timing of flashing and liquid-nitrogen filling. These programs are especially useful for efficient application of the system to single particle cryo-EM.
External linksJ Struct Biol / PubMed:30991102
MethodsEM (single particle)
Resolution1.9 Å
Structure data

EMDB-9890:
Cryo-EM structure of human apoferritin at 1.9A resolution by CRYO ARM 300
Method: EM (single particle) / Resolution: 1.9 Å

Source
  • Homo sapiens (human)

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