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TitleMorphologies of synaptic protein membrane fusion interfaces.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 114, Issue 34, Page 9110-9115, Year 2017
Publish dateAug 22, 2017
AuthorsPreeti Gipson / Yoshiyuki Fukuda / Radostin Danev / Ying Lai / Dong-Hua Chen / Wolfgang Baumeister / Axel T Brunger /
PubMed AbstractNeurotransmitter release is orchestrated by synaptic proteins, such as SNAREs, synaptotagmin, and complexin, but the molecular mechanisms remain unclear. We visualized functionally active synaptic ...Neurotransmitter release is orchestrated by synaptic proteins, such as SNAREs, synaptotagmin, and complexin, but the molecular mechanisms remain unclear. We visualized functionally active synaptic proteins reconstituted into proteoliposomes and their interactions in a native membrane environment by electron cryotomography with a Volta phase plate for improved resolvability. The images revealed individual synaptic proteins and synaptic protein complex densities at prefusion contact sites between membranes. We observed distinct morphologies of individual synaptic proteins and their complexes. The minimal system, consisting of neuronal SNAREs and synaptotagmin-1, produced point and long-contact prefusion states. Morphologies and populations of these states changed as the regulatory factors complexin and Munc13 were added. Complexin increased the membrane separation, along with a higher propensity of point contacts. Further inclusion of the priming factor Munc13 exclusively restricted prefusion states to point contacts, all of which efficiently fused upon Ca triggering. We conclude that synaptic proteins have evolved to limit possible contact site assemblies and morphologies to those that promote fast Ca-triggered release.
External linksProc Natl Acad Sci U S A / PubMed:28739947 / PubMed Central
MethodsEM (tomography)
Structure data

EMDB-8807:
Volta phase plate tomogram of SV + PM vesicle mixture
Method: EM (tomography)

EMDB-8808:
Volta phase plate tomogram of SV(Cpx) + PM vesicle mixture
Method: EM (tomography)

EMDB-8809:
Volta phase plate tomogram of SV(Cpx) + PM(C1C2BMUN) vesicle mixture
Method: EM (tomography)

EMDB-8810:
Volta phase plate tomogram of SV(Cpx) + PM + Ca2+ vesicle mixture
Method: EM (tomography)

EMDB-8811:
Volta phase plate tomogram of SV(Cpx) + PM(C1C2BMUN) + Ca2+ vesicle mixture
Method: EM (tomography)

Source
  • Rattus norvegicus (Norway rat)

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