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| Title | Trajectories of the ribosome as a Brownian nanomachine. |
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| Journal, issue, pages | Proc Natl Acad Sci U S A, Vol. 111, Issue 49, Page 17492-17497, Year 2014 |
| Publish date | Dec 9, 2014 |
 Authors | Ali Dashti / Peter Schwander / Robert Langlois / Russell Fung / Wen Li / Ahmad Hosseinizadeh / Hstau Y Liao / Jesper Pallesen / Gyanesh Sharma / Vera A Stupina / Anne E Simon / Jonathan D Dinman / Joachim Frank / Abbas Ourmazd /  |
| PubMed Abstract | A Brownian machine, a tiny device buffeted by the random motions of molecules in the environment, is capable of exploiting these thermal motions for many of the conformational changes in its work ...A Brownian machine, a tiny device buffeted by the random motions of molecules in the environment, is capable of exploiting these thermal motions for many of the conformational changes in its work cycle. Such machines are now thought to be ubiquitous, with the ribosome, a molecular machine responsible for protein synthesis, increasingly regarded as prototypical. Here we present a new analytical approach capable of determining the free-energy landscape and the continuous trajectories of molecular machines from a large number of snapshots obtained by cryogenic electron microscopy. We demonstrate this approach in the context of experimental cryogenic electron microscope images of a large ensemble of nontranslating ribosomes purified from yeast cells. The free-energy landscape is seen to contain a closed path of low energy, along which the ribosome exhibits conformational changes known to be associated with the elongation cycle. Our approach allows model-free quantitative analysis of the degrees of freedom and the energy landscape underlying continuous conformational changes in nanomachines, including those important for biological function. |
 External links | Proc Natl Acad Sci U S A / PubMed:25422471 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 20.0 Å |
| Structure data |  EMDB-6044: |
| Source |
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Saccharomyces cerevisiae (brewer's yeast)