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TitleInitial evaluation of a direct detection device detector for single particle cryo-electron microscopy.
Journal, issue, pagesJ Struct Biol, Vol. 176, Issue 3, Page 404-408, Year 2011
Publish dateSep 10, 2011
AuthorsAnna-Clare Milazzo / Anchi Cheng / Arne Moeller / Dmitry Lyumkis / Erica Jacovetty / James Polukas / Mark H Ellisman / Nguyen-Huu Xuong / Bridget Carragher / Clinton S Potter /
PubMed AbstractWe report on initial results of using a new direct detection device (DDD) for single particle reconstruction of vitreous ice embedded specimens. Images were acquired on a Tecnai F20 at 200keV and a ...We report on initial results of using a new direct detection device (DDD) for single particle reconstruction of vitreous ice embedded specimens. Images were acquired on a Tecnai F20 at 200keV and a nominal magnification of 29,000×. This camera has a significantly improved signal to noise ratio and modulation transfer function (MTF) at 200keV compared to a standard CCD camera installed on the same microscope. Control of the DDD has been integrated into Leginon, an automated data collection system. Using GroEL as a test specimen, we obtained images of ∼30K particles with the CCD and the DDD from the same specimen sample using essentially identical imaging conditions. Comparison of the maps reconstructed from the CCD images and the DDD images demonstrates the improved performance of the DDD. We also obtained a 3D reconstruction from ∼70K GroEL particles acquired using the DDD; the quality of the density map demonstrates the potential of this new recording device for cryoEM data acquisition.
External linksJ Struct Biol / PubMed:21933715 / PubMed Central
MethodsEM (single particle)
Resolution6.1 - 7.3 Å
Structure data

EMDB-5336:
Unprocessed map of GroEL obtained with a CCD camera to compare results from cryo SPA with a DDD camera using the same grid. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Method: EM (single particle) / Resolution: 7.3 Å

EMDB-5337:
Unprocessed map of GroEL obtained with a DDD camera to compare results from cryo SPA with a CCD camera using the same grid. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-5338:
High resolution map of GroEL obtained with a DDD camera. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Method: EM (single particle) / Resolution: 6.1 Å

EMDB-5339:
Amplitude corrected and filtered map of GroEL obtained with a DDD camera to compare results from cryo SPA with a CCD camera using the same grid. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-5340:
Processed map of GroEL obtained with a CCD camera to compare results from cryo SPA with a DDD camera using the same grid. Initial evaluation of a Direct Detection Device detector for single particle cryo-electron microscopy.
Method: EM (single particle) / Resolution: 7.3 Å

Source
  • Escherichia coli (E. coli)

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