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TitleMechanism of head-to-head MCM double-hexamer formation revealed by cryo-EM.
Journal, issue, pagesNature, Vol. 575, Issue 7784, Page 704-710, Year 2019
Publish dateNov 20, 2019
AuthorsThomas C R Miller / Julia Locke / Julia F Greiwe / John F X Diffley / Alessandro Costa /
PubMed AbstractIn preparation for bidirectional DNA replication, the origin recognition complex (ORC) loads two hexameric MCM helicases to form a head-to-head double hexamer around DNA. The mechanism of MCM double- ...In preparation for bidirectional DNA replication, the origin recognition complex (ORC) loads two hexameric MCM helicases to form a head-to-head double hexamer around DNA. The mechanism of MCM double-hexamer formation is debated. Single-molecule experiments have suggested a sequential mechanism, in which the ORC-dependent loading of the first hexamer drives the recruitment of the second hexamer. By contrast, biochemical data have shown that two rings are loaded independently via the same ORC-mediated mechanism, at two inverted DNA sites. Here we visualize MCM loading using time-resolved electron microscopy, and identify intermediates in the formation of the double hexamer. We confirm that both hexamers are recruited via the same interaction that occurs between ORC and the C-terminal domains of the MCM helicases. Moreover, we identify the mechanism of coupled MCM loading. The loading of the first MCM hexamer around DNA creates a distinct interaction site, which promotes the engagement of ORC at the N-terminal homodimerization interface of MCM. In this configuration, ORC is poised to direct the recruitment of the second hexamer in an inverted orientation, which is suitable for the formation of the double hexamer. Our results therefore reconcile the two apparently contrasting models derived from single-molecule experiments and biochemical data.
External linksNature / PubMed:31748745 / PubMed Central
MethodsEM (single particle)
Resolution4.4 Å
Structure data

4980

6rqc

EMDB-4980, PDB-6rqc:
Cryo-EM structure of an MCM loading intermediate
Method: EM (single particle) / Resolution: 4.4 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-ZN:
Unknown entry

Source
  • saccharomyces cerevisiae s288c (yeast)
KeywordsREPLICATION / DNA Replication / Origin licensing / MCM2-7 helicase / Origin Recognition Complex

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