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TitleConformational proofreading of distant 40S ribosomal subunit maturation events by a long-range communication mechanism.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 2754, Year 2019
Publish dateJun 21, 2019
AuthorsValentin Mitterer / Ramtin Shayan / Sébastien Ferreira-Cerca / Guillaume Murat / Tanja Enne / Dana Rinaldi / Sarah Weigl / Hajrija Omanic / Pierre-Emmanuel Gleizes / Dieter Kressler / Celia Plisson-Chastang / Brigitte Pertschy /
PubMed AbstractEukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is ...Eukaryotic ribosomes are synthesized in a hierarchical process driven by a plethora of assembly factors, but how maturation events at physically distant sites on pre-ribosomes are coordinated is poorly understood. Using functional analyses and cryo-EM, we show that ribosomal protein Rps20 orchestrates communication between two multi-step maturation events across the pre-40S subunit. Our study reveals that during pre-40S maturation, formation of essential contacts between Rps20 and Rps3 permits assembly factor Ltv1 to recruit the Hrr25 kinase, thereby promoting Ltv1 phosphorylation. In parallel, a deeply buried Rps20 loop reaches to the opposite pre-40S side, where it stimulates Rio2 ATPase activity. Both cascades converge to the final maturation steps releasing Rio2 and phosphorylated Ltv1. We propose that conformational proofreading exerted via Rps20 constitutes a checkpoint permitting assembly factor release and progression of pre-40S maturation only after completion of all earlier maturation steps.
External linksNat Commun / PubMed:31227701 / PubMed Central
MethodsEM (single particle)
Resolution3.15 - 3.8 Å
Structure data

4792

6rbd

6rbe

EMDB-4792, PDB-6rbd:
State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
Method: EM (single particle) / Resolution: 3.47 Å

4793

6rbd

6rbe

EMDB-4793, PDB-6rbe:
State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
Method: EM (single particle) / Resolution: 3.8 Å

4794

6rbd

6rbe

EMDB-4794: Yeast pre-40S Rps20 DeltaLoop C1-Headonly
PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
Method: EM (single particle) / Resolution: 3.8 Å

4795

6rbd

6rbe

EMDB-4795: Yeast pre-40S Rps20 DeltaLoop C2-Headonly
PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
Method: EM (single particle) / Resolution: 3.8 Å

4796

6rbd

6rbe

EMDB-4796: yeast pre-40S particle - focus on Dim1
PDB-6rbd: State 1 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
PDB-6rbe: State 2 of yeast Tsr1-TAP Rps20-Deltaloop pre-40S particles
Method: EM (single particle) / Resolution: 3.15 Å

Source
  • saccharomyces cerevisiae s288c (yeast)
  • Baker's yeast (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsRIBOSOME / pre-40S / yeast / cytoplasmic / mutant Rps20

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