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Structure paper

TitleStructural determinants of microtubule minus end preference in CAMSAP CKK domains.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 5236, Year 2019
Publish dateNov 20, 2019
AuthorsJoseph Atherton / Yanzhang Luo / Shengqi Xiang / Chao Yang / Ankit Rai / Kai Jiang / Marcel Stangier / Annapurna Vemu / Alexander D Cook / Su Wang / Antonina Roll-Mecak / Michel O Steinmetz / Anna Akhmanova / Marc Baldus / Carolyn A Moores /
PubMed AbstractCAMSAP/Patronins regulate microtubule minus-end dynamics. Their end specificity is mediated by their CKK domains, which we proposed recognise specific tubulin conformations found at minus ends. To ...CAMSAP/Patronins regulate microtubule minus-end dynamics. Their end specificity is mediated by their CKK domains, which we proposed recognise specific tubulin conformations found at minus ends. To critically test this idea, we compared the human CAMSAP1 CKK domain (HsCKK) with a CKK domain from Naegleria gruberi (NgCKK), which lacks minus-end specificity. Here we report near-atomic cryo-electron microscopy structures of HsCKK- and NgCKK-microtubule complexes, which show that these CKK domains share the same protein fold, bind at the intradimer interprotofilament tubulin junction, but exhibit different footprints on microtubules. NMR experiments show that both HsCKK and NgCKK are remarkably rigid. However, whereas NgCKK binding does not alter the microtubule architecture, HsCKK remodels its microtubule interaction site and changes the underlying polymer structure because the tubulin lattice conformation is not optimal for its binding. Thus, in contrast to many MAPs, the HsCKK domain can differentiate subtly specific tubulin conformations to enable microtubule minus-end recognition.
External linksNat Commun / PubMed:31748546 / PubMed Central
MethodsEM (single particle)
Resolution3.7 - 3.8 Å
Structure data

4643

6qus

EMDB-4643, PDB-6qus:
HsCKK (human CAMSAP1) decorated 13pf taxol-GDP microtubule
Method: EM (single particle) / Resolution: 3.7 Å

4644

6quy

EMDB-4644, PDB-6quy:
NgCKK (N.Gruberi CKK) decorated 13pf taxol-GDP microtubule
Method: EM (single particle) / Resolution: 3.8 Å

4650

6qve

EMDB-4650, PDB-6qve:
NgCKK (Naegleria Gruberi CKK) decorated 14pf taxol-GDP microtubule
Method: EM (single particle) / Resolution: 3.7 Å

4654

6qvj

EMDB-4654, PDB-6qvj:
HsCKK (human CAMSAP1) decorated 14pf taxol-GDP microtubule
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-TA1:
TAXOL / medication, chemotherapy*YM / Paclitaxel

Source
  • homo sapiens (human)
  • Human (human)
  • naegleria gruberi (eukaryote)
KeywordsSTRUCTURAL PROTEIN / Microtubule CAMSAP Calmodulin-regulated spectrum-associated proteins CKK Cryo-EM Cryo-Electron Microscopy

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