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Title and high-resolution cryo-EM structure of a bacterial type VI secretion system membrane complex.
Journal, issue, pagesEMBO J, Vol. 38, Issue 10, Year 2019
Publish dateMay 15, 2019
AuthorsChiara Rapisarda / Yassine Cherrak / Romain Kooger / Victoria Schmidt / Riccardo Pellarin / Laureen Logger / Eric Cascales / Martin Pilhofer / Eric Durand / Rémi Fronzes /
PubMed AbstractBacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is ...Bacteria have evolved macromolecular machineries that secrete effectors and toxins to survive and thrive in diverse environments. The type VI secretion system (T6SS) is a contractile machine that is related to phages. It is composed of a phage tail-like structure inserted in the bacterial cell envelope by a membrane complex (MC) comprising the TssJ, TssL and TssM proteins. We previously reported the low-resolution negative-stain electron microscopy structure of the enteroaggregative MC and proposed a rotational 5-fold symmetry with a TssJ:TssL:TssM stoichiometry of 2:2:2. Here, cryo-electron tomography analyses of the T6SS MC confirm the 5-fold symmetry and identify the regions of the structure that insert into the bacterial membranes. A high-resolution model obtained by single-particle cryo-electron microscopy highlights new features: five additional copies of TssJ, yielding a TssJ:TssL:TssM stoichiometry of 3:2:2, an 11-residue loop in TssM, protruding inside the lumen of the MC and constituting a functionally important periplasmic gate, and hinge regions. Based on these data, we propose an updated model on MC structure and dynamics during T6SS assembly and function.
External linksEMBO J / PubMed:30877094 / PubMed Central
MethodsEM (single particle) / EM (subtomogram averaging)
Resolution4.6 - 25.0 Å
Structure data

0264

6hs7

EMDB-0264, PDB-6hs7:
Type VI membrane complex
Method: EM (single particle) / Resolution: 4.6 Å

EMDB-0265:
Type VI membrane complex
Method: EM (single particle) / Resolution: 4.9 Å

EMDB-0266:
Type VI membrane complex
Method: EM (single particle) / Resolution: 7.9 Å

EMDB-0267:
Type VI secretion membrane complex
Method: EM (single particle) / Resolution: 17.0 Å

EMDB-4561:
STA of TssJLM
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

EMDB-4562:
Subtomogram average of T6SS membrane complex TssJLM
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

Source
  • escherichia coli (E. coli)
  • Escherichia coli 042 (bacteria)
KeywordsMEMBRANE PROTEIN / Membrane complex / tether

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