Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Square beams for optimal tiling in transmission electron microscopy. |
|---|---|
| Journal, issue, pages | Nat Methods, Vol. 21, Issue 4, Page 562-565, Year 2024 |
| Publish date | Jan 18, 2024 |
 Authors | Eugene Y D Chua / Lambertus M Alink / Mykhailo Kopylov / Jake D Johnston / Fabian Eisenstein / Alex de Marco /   |
| PubMed Abstract | Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect ...Imaging large fields of view at a high magnification requires tiling. Transmission electron microscopes typically have round beam profiles; therefore, tiling across a large area is either imperfect or results in uneven exposures, a problem for dose-sensitive samples. Here, we introduce a square electron beam that can easily be retrofitted in existing microscopes, and demonstrate its application, showing that it can tile nearly perfectly and deliver cryo-electron microscopy imaging with a resolution comparable to conventional set-ups. |
 External links | Nat Methods / PubMed:38238558 / PubMed Central |
| Methods | EM (single particle) / EM (tomography) |
| Resolution | 2.06 - 2.32 Å |
| Structure data |  EMDB-42371: Mouse apoferritin imaged with a square aperture  EMDB-42372: Mouse apoferritin imaged with a round aperture  EMDB-42373: Mouse apoferritin imaged with a square aperture with P2 projection lens rotation, reconstructed from 25,000 particles  EMDB-42374: Mouse apoferritin imaged with a round aperture without P2 projection lens rotation, reconstructed from 25,000 particles  EMDB-42851: 5x5 tiled montage tomogram of a holey carbon grid with apoferritin imaged with a square electron beam  EMDB-42879: 3x3 tiled montage tomogram of a yeast lamella imaged with a square electron beam |
| Source |
|
Mus musculus (house mouse)
