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TitleStructural basis of U12-type intron engagement by the fully assembled human minor spliceosome.
Journal, issue, pagesScience, Vol. 383, Issue 6688, Page 1245-1252, Year 2024
Publish dateMar 15, 2024
AuthorsRui Bai / Meng Yuan / Pu Zhang / Ting Luo / Yigong Shi / Ruixue Wan /
PubMed AbstractThe minor spliceosome, which is responsible for the splicing of U12-type introns, comprises five small nuclear RNAs (snRNAs), of which only one is shared with the major spliceosome. In this work, we ...The minor spliceosome, which is responsible for the splicing of U12-type introns, comprises five small nuclear RNAs (snRNAs), of which only one is shared with the major spliceosome. In this work, we report the 3.3-angstrom cryo-electron microscopy structure of the fully assembled human minor spliceosome pre-B complex. The atomic model includes U11 small nuclear ribonucleoprotein (snRNP), U12 snRNP, and U4atac/U6atac.U5 tri-snRNP. U11 snRNA is recognized by five U11-specific proteins (20K, 25K, 35K, 48K, and 59K) and the heptameric Sm ring. The 3' half of the 5'-splice site forms a duplex with U11 snRNA; the 5' half is recognized by U11-35K, U11-48K, and U11 snRNA. Two proteins, CENATAC and DIM2/TXNL4B, specifically associate with the minor tri-snRNP. A structural analysis uncovered how two conformationally similar tri-snRNPs are differentiated by the minor and major prespliceosomes for assembly.
External linksScience / PubMed:38484052
MethodsEM (single particle)
Resolution3.13 - 4.66 Å
Structure data

38993

8y6o

EMDB-38993, PDB-8y6o:
Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U11 and tri-snRNP part
Method: EM (single particle) / Resolution: 3.38 Å

EMDB-38999: Focused refinement map of BRR2 region of the human minor pre-B complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-39000: focused refinement map for the U11 snRNP in the human minor pre-B complex
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-39005: Focused refinement map of U5 snRNP of the human minor pre-B complex
Method: EM (single particle) / Resolution: 3.13 Å

EMDB-39006: Focused refinement map of U5 Sm ring region of the human minor pre-B complex
Method: EM (single particle) / Resolution: 3.19 Å

EMDB-39007: Focused refinement map for U4atac/U6atac region of the human minor pre-B complex
Method: EM (single particle) / Resolution: 3.6 Å

39013

8y7e

EMDB-39013, PDB-8y7e:
Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U12 snRNP part
Method: EM (single particle) / Resolution: 4.66 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-IHP:
INOSITOL HEXAKISPHOSPHATE / Phytic acid

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
KeywordsSPLICING/RNA / spliceosome / minor spliceosome / U11 snRNP / U12 snRNP / U4atac / U6atac / CENATAC / TXNL4B / U11 snRNA / minor tri-snRNP / U12-type intron / SPLICING-RNA complex

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