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TitleStructure and function of the ABC transporter ABCB19 in brassinosteroid export.
Journal, issue, pagesScience, Vol. 383, Issue 6689, Page eadj4591, Year 2024
Publish dateMar 22, 2024
AuthorsWei Ying / Yaowei Wang / Hong Wei / Yongming Luo / Qian Ma / Heyuan Zhu / Hilde Janssens / Nemanja Vukašinović / Miroslav Kvasnica / Johan M Winne / Yongxiang Gao / Shutang Tan / Jiří Friml / Xin Liu / Eugenia Russinova / Linfeng Sun /
PubMed AbstractBrassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but ...Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. Brassinosteroids are synthesized in the cell interior but bind receptors at the cell surface, necessitating a yet to be identified export mechanism. Here, we show that a member of the ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as a brassinosteroid exporter. We present its structure in both the substrate-unbound and the brassinosteroid-bound states. Bioactive brassinosteroids are potent activators of ABCB19 ATP hydrolysis activity, and transport assays showed that ABCB19 transports brassinosteroids. In , ABCB19 and its close homolog, ABCB1, positively regulate brassinosteroid responses. Our results uncover an elusive export mechanism for bioactive brassinosteroids that is tightly coordinated with brassinosteroid signaling.
External linksScience / PubMed:38513023
MethodsEM (single particle)
Resolution3.4 - 3.9 Å
Structure data

37690

8woi

EMDB-37690, PDB-8woi:
Structure of the wild-type Arabidopsis ABCB19 in the apo state
Method: EM (single particle) / Resolution: 3.4 Å

37692

8wom

EMDB-37692, PDB-8wom:
Structure of the wild-type Arabidopsis ABCB19 in the brassinolide-bound state
Method: EM (single particle) / Resolution: 3.5 Å

37694

8woo

EMDB-37694, PDB-8woo:
Structure of the wild-type Arabidopsis ABCB19 in the brassinolide and AMP-PNP bound state
Method: EM (single particle) / Resolution: 3.9 Å

37705

8wp0

EMDB-37705, PDB-8wp0:
Structure of the Arabidopsis E529Q/E1174Q ABCB19 in the ATP bound state
Method: EM (single particle) / Resolution: 3.4 Å

Chemicals

ChemComp-BLD:
Brassinolide / hormone*YM / Brassinolide

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / ABC transporter / wild-type / apo state / brassinolide-bound state

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