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TitleStructure of the intact tail machine of Anabaena myophage A-1(L).
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 2654, Year 2024
Publish dateMar 26, 2024
AuthorsRong-Cheng Yu / Feng Yang / Hong-Yan Zhang / Pu Hou / Kang Du / Jie Zhu / Ning Cui / Xudong Xu / Yuxing Chen / Qiong Li / Cong-Zhao Zhou /
PubMed AbstractThe Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution ...The Myoviridae cyanophage A-1(L) specifically infects the model cyanobacteria Anabaena sp. PCC 7120. Following our recent report on the capsid structure of A-1(L), here we present the high-resolution cryo-EM structure of its intact tail machine including the neck, tail and attached fibers. Besides the dodecameric portal, the neck contains a canonical hexamer connected to a unique pentadecamer that anchors five extended bead-chain-like neck fibers. The 1045-Å-long contractile tail is composed of a helical bundle of tape measure proteins surrounded by a layer of tube proteins and a layer of sheath proteins, ended with a five-component baseplate. The six long and six short tail fibers are folded back pairwise, each with one end anchoring to the baseplate and the distal end pointing to the capsid. Structural analysis combined with biochemical assays further enable us to identify the dual hydrolytic activities of the baseplate hub, in addition to two host receptor binding domains in the tail fibers. Moreover, the structure of the intact A-1(L) also helps us to reannotate its genome. These findings will facilitate the application of A-1(L) as a chassis cyanophage in synthetic biology.
External linksNat Commun / PubMed:38531972 / PubMed Central
MethodsEM (single particle)
Resolution3.26 - 3.9 Å
Structure data

37150

8ke9

EMDB-37150, PDB-8ke9:
The CBD domain of cyanophage A-1(L) short tail fiber
Method: EM (single particle) / Resolution: 3.76 Å

37151

8kea

EMDB-37151, PDB-8kea:
Cyanophage A-1(L) baseplate-initiators
Method: EM (single particle) / Resolution: 3.44 Å

37152

8kec

EMDB-37152, PDB-8kec:
Cyanophage A-1(L) tail fiber
Method: EM (single particle) / Resolution: 3.9 Å

37153

8kee

EMDB-37153, PDB-8kee:
Cyanophage A-1(L) sheath-tube
Method: EM (single particle) / Resolution: 3.26 Å

37154

8kef

EMDB-37154, PDB-8kef:
Cyanophage A-1(L) neck/gp7-terminator
Method: EM (single particle) / Resolution: 3.44 Å

37155

8keg

EMDB-37155, PDB-8keg:
Cyanophage A-1(L) neck/gp5-neck fiber
Method: EM (single particle) / Resolution: 3.66 Å

41590

8ts6

EMDB-41590, PDB-8ts6:
Cyanophage A-1(L) portal
Method: EM (single particle) / Resolution: 3.44 Å

Source
  • unclassified caudoviricetes (virus)
  • uncultured cyanophage (environmental samples)
KeywordsVIRAL PROTEIN / fiber / virus / baseplate / tail / neck / portal

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