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TitleMetallo-supramolecular branched polymer protects particles from air-water interface in single-particle cryo-electron microscopy.
Journal, issue, pagesCommun Biol, Vol. 7, Issue 1, Page 65, Year 2024
Publish dateJan 9, 2024
AuthorsYixin Xu / Yuqi Qin / Lang Wang / Yingyi Zhang / Yufeng Wang / Shangyu Dang /
PubMed AbstractRecent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current ...Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck in the current single particle cryo-EM pipeline is the preparation of good quality frozen cryo-EM grids, which is mostly a trial-and-error process. Among many issues, preferred particle orientation and sample damage by air-water interface (AWI) are common practical problems. Here we report a method of applying metallo-supramolecular branched polymer (MSBP) in the cryo-sample preparation for high-resolution single-particle cryo-EM. Our data shows that MSBP keeps a majority of particles away from air-water interface and mitigates preferred orientation as verified by the analyses of apoferritin, hemagglutinin) trimer and various sample proteins. The use of MSBP is a simple method to improve particle distribution for high-resolution structure determination in single-particle cryo-EM.
External linksCommun Biol / PubMed:38195919 / PubMed Central
MethodsEM (single particle)
Resolution2.16 - 3.19 Å
Structure data

EMDB-36313: Cryo-EM structure of apoferritin with MSBP
Method: EM (single particle) / Resolution: 2.16 Å

EMDB-36314: Cryo-EM structure of hemagglutinin with MSBP
Method: EM (single particle) / Resolution: 3.19 Å

EMDB-36315: Cryo-EM structure of catalase with MSBP
Method: EM (single particle) / Resolution: 2.97 Å

EMDB-36316: Cryo-EM structure of beta-Galactosidase with MSBP
Method: EM (single particle) / Resolution: 3.18 Å

Source
  • Mus musculus (house mouse)
  • Influenza A virus
  • Homo sapiens (human)
  • Escherichia coli (E. coli)

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