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TitleCryo-EM structures of the plant anion channel SLAC1 from Arabidopsis thaliana suggest a combined activation model.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 7345, Year 2023
Publish dateNov 14, 2023
AuthorsYeongmok Lee / Hyeon Seong Jeong / Seoyeon Jung / Junmo Hwang / Chi Truc Han Le / Sung-Hoon Jun / Eun Jo Du / KyeongJin Kang / Beom-Gi Kim / Hyun-Ho Lim / Sangho Lee /
PubMed AbstractThe anion channel SLAC1 functions as a crucial effector in the ABA signaling, leading to stomata closure. SLAC1 is activated by phosphorylation in its intracellular domains. Both a binding-activation ...The anion channel SLAC1 functions as a crucial effector in the ABA signaling, leading to stomata closure. SLAC1 is activated by phosphorylation in its intracellular domains. Both a binding-activation model and an inhibition-release model for activation have been proposed based on only the closed structures of SLAC1, rendering the structure-based activation mechanism controversial. Here we report cryo-EM structures of Arabidopsis SLAC1 WT and its phosphomimetic mutants in open and closed states. Comparison of the open structure with the closed ones reveals the structural basis for opening of the conductance pore. Multiple phosphorylation of an intracellular domain (ICD) causes dissociation of ICD from the transmembrane domain. A conserved, positively-charged sequence motif in the intracellular loop 2 (ICL2) seems to be capable of sensing of the negatively charged phosphorylated ICD. Interactions between ICL2 and ICD drive drastic conformational changes, thereby widening the pore. From our results we propose that SLAC1 operates by a mechanism combining the binding-activation and inhibition-release models.
External linksNat Commun / PubMed:37963863 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 3.84 Å
Structure data

34303

8gw6

EMDB-34303, PDB-8gw6:
AtSLAC1 6D mutant in closed state
Method: EM (single particle) / Resolution: 3.3 Å

34304

8gw7

EMDB-34304, PDB-8gw7:
AtSLAC1 6D mutant in open state
Method: EM (single particle) / Resolution: 3.3 Å

35904

8j0j

EMDB-35904, PDB-8j0j:
AtSLAC1 8D mutant in closed state
Method: EM (single particle) / Resolution: 2.7 Å

35920

8j1e

EMDB-35920, PDB-8j1e:
AtSLAC1 in open state
Method: EM (single particle) / Resolution: 3.84 Å

Chemicals

ChemComp-CL:
Unknown entry / Chloride

ChemComp-Y01:
CHOLESTEROL HEMISUCCINATE

Source
  • arabidopsis thaliana (thale cress)
  • aequorea victoria (jellyfish)
KeywordsMEMBRANE PROTEIN / Stomatal closure / anion channel / phosphorylation-dependent activation

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