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Structure paper

TitleStructures and mechanisms of the Arabidopsis cytokinin transporter AZG1.
Journal, issue, pagesNat Plants, Vol. 10, Issue 1, Page 180-191, Year 2024
Publish dateJan 3, 2024
AuthorsLingyi Xu / Wei Jia / Xin Tao / Fan Ye / Yan Zhang / Zhong Jie Ding / Shao Jian Zheng / Shuai Qiao / Nannan Su / Yu Zhang / Shan Wu / Jiangtao Guo /
PubMed AbstractCytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine ...Cytokinins are essential for plant growth and development, and their tissue distributions are regulated by transmembrane transport. Recent studies have revealed that members of the 'Aza-Guanine Resistant' (AZG) protein family from Arabidopsis thaliana can mediate cytokinin uptake in roots. Here we present 2.7 to 3.3 Å cryo-electron microscopy structures of Arabidopsis AZG1 in the apo state and in complex with its substrates trans-zeatin (tZ), 6-benzyleaminopurine (6-BAP) or kinetin. AZG1 forms a homodimer and each subunit shares a similar topology and domain arrangement with the proteins of the nucleobase/ascorbate transporter (NAT) family. These structures, along with functional analyses, reveal the molecular basis for cytokinin recognition. Comparison of the AZG1 structures determined in inward-facing conformations and predicted by AlphaFold2 in the occluded conformation allowed us to propose that AZG1 may carry cytokinins across the membrane through an elevator mechanism.
External linksNat Plants / PubMed:38172575
MethodsEM (single particle)
Resolution2.6 - 3.3 Å
Structure data

35678

8irl

EMDB-35678, PDB-8irl:
Apo state of Arabidopsis AZG1 at pH 7.4
Method: EM (single particle) / Resolution: 2.7 Å

35679

8irm

EMDB-35679, PDB-8irm:
Endogenous substrate adenine bound state of Arabidopsis AZG1 at pH 5.5
Method: EM (single particle) / Resolution: 2.6 Å

35680

8irn

EMDB-35680, PDB-8irn:
6-BAP bound state of Arabidopsis AZG1
Method: EM (single particle) / Resolution: 2.7 Å

35681

8iro

EMDB-35681, PDB-8iro:
trans-Zeatin bound state of Arabidopsis AZG1 at pH7.4
Method: EM (single particle) / Resolution: 2.7 Å

35682

8irp

EMDB-35682, PDB-8irp:
kinetin bound state of Arabidopsis AZG1
Method: EM (single particle) / Resolution: 2.8 Å

37658

8wmq

EMDB-37658, PDB-8wmq:
trans-Zeatin bound state of Arabidopsis AZG1 at pH5.5
Method: EM (single particle) / Resolution: 3.3 Å

37681

8wo7

EMDB-37681, PDB-8wo7:
Apo state of Arabidopsis AZG1 T440Y
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-ADE:
ADENINE / Adenine

ChemComp-HOH:
WATER / Water

ChemComp-EMU:
N-BENZYL-9H-PURIN-6-AMINE / inhibitor*YM / 6-Benzylaminopurine

ChemComp-ZEA:
(2E)-2-methyl-4-(9H-purin-6-ylamino)but-2-en-1-ol / Zeatin

ChemComp-H35:
N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE / hormone*YM / Kinetin

Source
  • arabidopsis thaliana (thale cress)
KeywordsTRANSPORT PROTEIN / cytokinin / transporter

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