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TitleCalaxin stabilizes the docking of outer arm dyneins onto ciliary doublet microtubule in vertebrates.
Journal, issue, pagesElife, Vol. 12, Year 2023
Publish dateApr 14, 2023
AuthorsHiroshi Yamaguchi / Motohiro Morikawa / Masahide Kikkawa /
PubMed AbstractOuter arm dynein (OAD) is the main force generator of ciliary beating. Although OAD loss is the most frequent cause of human primary ciliary dyskinesia, the docking mechanism of OAD onto the ciliary ...Outer arm dynein (OAD) is the main force generator of ciliary beating. Although OAD loss is the most frequent cause of human primary ciliary dyskinesia, the docking mechanism of OAD onto the ciliary doublet microtubule (DMT) remains elusive in vertebrates. Here, we analyzed the functions of Calaxin/Efcab1 and Armc4, the two of five components of vertebrate OAD-DC (docking complex), using zebrafish spermatozoa and cryo-electron tomography. Mutation of caused complete loss of OAD, whereas mutation of caused only partial loss of OAD. Detailed structural analysis revealed that OADs are tethered to DMT through DC components other than Calaxin, and that recombinant Calaxin can autonomously rescue the deficient DC structure and the OAD instability. Our data demonstrate the discrete roles of Calaxin and Armc4 in the OAD-DMT interaction, suggesting the stabilizing process of OAD docking onto DMT in vertebrates.
External linksElife / PubMed:37057896 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution18.1 - 23.6 Å
Structure data

EMDB-34791: Doublet microtubule of zebrafish sperm axoneme, WT
Method: EM (subtomogram averaging) / Resolution: 22.0 Å

EMDB-34792: Radial spoke 1 of zebrafish sperm axoneme, WT
Method: EM (subtomogram averaging) / Resolution: 21.4 Å

EMDB-34793: Radial spoke 2 of zebrafish sperm axoneme, WT
Method: EM (subtomogram averaging) / Resolution: 21.4 Å

EMDB-34794: Radial spoke 3 of zebrafish sperm axoneme, WT
Method: EM (subtomogram averaging) / Resolution: 21.7 Å

EMDB-34795: Doublet microtubule of zebrafish sperm axoneme, calaxin-/-, OAD+ class
Method: EM (subtomogram averaging) / Resolution: 22.6 Å

EMDB-34796: Doublet microtubule of zebrafish sperm axoneme, calaxin-/-, OAD- class
Method: EM (subtomogram averaging) / Resolution: 23.5 Å

EMDB-34797: Doublet microtubule of zebrafish sperm axoneme, armc4-/-
Method: EM (subtomogram averaging) / Resolution: 23.6 Å

EMDB-34798: Outer arm dynein of zebrafish sperm axoneme, WT
Method: EM (subtomogram averaging) / Resolution: 18.1 Å

EMDB-34799: Outer arm dynein of zebrafish sperm axoneme, WT, refined focusing on the docking complex
Method: EM (subtomogram averaging) / Resolution: 18.5 Å

EMDB-34800: Outer arm dynein of zebrafish sperm axoneme, WT, 1 mM calcium condition, refined focusing on the docking complex
Method: EM (subtomogram averaging) / Resolution: 19.7 Å

EMDB-34801: Outer arm dynein of zebrafish sperm axoneme, calaxin-/-, refined focusing on the docking complex
Method: EM (subtomogram averaging) / Resolution: 18.6 Å

EMDB-34802: Outer arm dynein of zebrafish sperm axoneme, calaxin-/-, incubated with recombinant Calaxin protein, refined focusing on the docking complex
Method: EM (subtomogram averaging) / Resolution: 20.0 Å

Source
  • Danio rerio (zebrafish)

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