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TitleStructure of the 40S-ABCE1 post-splitting complex in ribosome recycling and translation initiation.
Journal, issue, pagesNat Struct Mol Biol, Vol. 24, Issue 5, Page 453-460, Year 2017
Publish dateApr 3, 2017
AuthorsAndré Heuer / Milan Gerovac / Christian Schmidt / Simon Trowitzsch / Anne Preis / Peter Kötter / Otto Berninghausen / Thomas Becker / Roland Beckmann / Robert Tampé /
PubMed AbstractThe essential ATP-binding cassette protein ABCE1 splits 80S ribosomes into 60S and 40S subunits after canonical termination or quality-control-based mRNA surveillance processes. However, the ...The essential ATP-binding cassette protein ABCE1 splits 80S ribosomes into 60S and 40S subunits after canonical termination or quality-control-based mRNA surveillance processes. However, the underlying splitting mechanism remains enigmatic. Here, we present a cryo-EM structure of the yeast 40S-ABCE1 post-splitting complex at 3.9-Å resolution. Compared to the pre-splitting state, we observe repositioning of ABCE1's iron-sulfur cluster domain, which rotates 150° into a binding pocket on the 40S subunit. This repositioning explains a newly observed anti-association activity of ABCE1. Notably, the movement implies a collision with A-site factors, thus explaining the splitting mechanism. Disruption of key interactions in the post-splitting complex impairs cellular homeostasis. Additionally, the structure of a native post-splitting complex reveals ABCE1 to be part of the 43S initiation complex, suggesting a coordination of termination, recycling, and initiation.
External linksNat Struct Mol Biol / PubMed:28368393
MethodsEM (single particle)
Resolution3.9 - 14.0 Å
Structure data

EMDB-3452:
native 40S-ABCE1 complex
Method: EM (single particle) / Resolution: 14.0 Å

4071

5ll6

EMDB-4071, PDB-5ll6:
Structure of the 40S ABCE1 post-splitting complex in ribosome recycling and translation initiation
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

Source
  • saccharomyces cerevisiae (brewer's yeast)
KeywordsRIBOSOME / ABCE1 / recycling / 40S

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