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TitleStructural convergence endows nuclear transport receptor Kap114p with a transcriptional repressor function toward TATA-binding protein.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 5518, Year 2023
Publish dateSep 8, 2023
AuthorsChung-Chi Liao / Yi-Sen Wang / Wen-Chieh Pi / Chun-Hsiung Wang / Yi-Min Wu / Wei-Yi Chen / Kuo-Chiang Hsia /
PubMed AbstractThe transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-β ...The transcription factor TATA-box binding protein (TBP) modulates gene expression in nuclei. This process requires the involvement of nuclear transport receptors, collectively termed karyopherin-β (Kap-β) in yeast, and various regulatory factors. In previous studies we showed that Kap114p, a Kap-β that mediates nuclear import of yeast TBP (yTBP), modulates yTBP-dependent transcription. However, how Kap114p associates with yTBP to exert its multifaceted functions has remained elusive. Here, we employ single-particle cryo-electron microscopy to determine the structure of Kap114p in complex with the core domain of yTBP (yTBP). Remarkably, Kap114p wraps around the yTBP N-terminal lobe, revealing a structure resembling transcriptional regulators in complex with TBP, suggesting convergent evolution of the two protein groups for a common function. We further demonstrate that Kap114p sequesters yTBP away from promoters, preventing a collapse of yTBP dynamics required for yeast responses to environmental stress. Hence, we demonstrate that nuclear transport receptors represent critical elements of the transcriptional regulatory network.
External linksNat Commun / PubMed:37684250 / PubMed Central
MethodsEM (single particle)
Resolution4.03 Å
Structure data

34490

8h5b

EMDB-34490, PDB-8h5b:
The cryo-EM structure of nuclear transport receptor Kap114p complex with yeast TATA-box binding protein
Method: EM (single particle) / Resolution: 4.03 Å

Source
  • Saccharomyces cerevisiae S228 (yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsPROTEIN TRANSPORT / nuclear transport receptor Kap114p / yeast TATA-box binding protein

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