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TitleTIR-catalyzed ADP-ribosylation reactions produce signaling molecules for plant immunity.
Journal, issue, pagesScience, Vol. 377, Issue 6605, Page eabq8180, Year 2022
Publish dateJul 29, 2022
AuthorsAolin Jia / Shijia Huang / Wen Song / Junli Wang / Yonggang Meng / Yue Sun / Lina Xu / Henriette Laessle / Jan Jirschitzka / Jiao Hou / Tiantian Zhang / Wenquan Yu / Giuliana Hessler / Ertong Li / Shoucai Ma / Dongli Yu / Jan Gebauer / Ulrich Baumann / Xiaohui Liu / Zhifu Han / Junbiao Chang / Jane E Parker / Jijie Chai /
PubMed AbstractPlant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease ...Plant pathogen-activated immune signaling by nucleotide-binding leucine-rich repeat (NLR) receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain converges on Enhanced Disease Susceptibility 1 (EDS1) and its direct partners, Phytoalexin Deficient 4 (PAD4) or Senescence-Associated Gene 101 (SAG101). TIR-encoded nicotinamide adenine dinucleotide hydrolase (NADase) produces signaling molecules to promote exclusive EDS1-PAD4 and EDS1-SAG101 interactions with helper NLR subclasses. In this work, we show that TIR-containing proteins catalyze adenosine diphosphate (ADP)-ribosylation of adenosine triphosphate (ATP) and ADP ribose (ADPR) through ADPR polymerase-like and NADase activity, forming ADP-ribosylated ATP (ADPr-ATP) and ADPr-ADPR (di-ADPR), respectively. Specific binding of ADPr-ATP or di-ADPR allosterically promotes EDS1-SAG101 interaction with helper NLR N requirement gene 1A (NRG1A) in vitro and in planta. Our data reveal an enzymatic activity of TIRs that enables specific activation of the EDS1-SAG101-NRG1 immunity branch.
External linksScience / PubMed:35857644
MethodsEM (single particle) / X-ray diffraction
Resolution2.52 - 2.71 Å
Structure data

33233

7xjp

EMDB-33233, PDB-7xjp:
Cryo-EM structure of EDS1 and SAG101 with ATP-APDR
Method: EM (single particle) / Resolution: 2.71 Å

PDB-7xoz:
Crystal structure of RPPT-TIR
Method: X-RAY DIFFRACTION / Resolution: 2.52 Å

Chemicals

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM / Isopropyl alcohol

ChemComp-APR:
ADENOSINE-5-DIPHOSPHORIBOSE

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-HOH:
WATER / Water

Source
  • arabidopsis (plant)
  • arabidopsis thaliana (thale cress)
KeywordsPLANT PROTEIN / NLR / Plant immune signaling / HYDROLASE

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