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TitleCullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome.
Journal, issue, pagesNature, Vol. 531, Issue 7596, Page 598-603, Year 2016
Publish dateMar 31, 2016
AuthorsSimone Cavadini / Eric S Fischer / Richard D Bunker / Alessandro Potenza / Gondichatnahalli M Lingaraju / Kenneth N Goldie / Weaam I Mohamed / Mahamadou Faty / Georg Petzold / Rohan E J Beckwith / Ritesh B Tichkule / Ulrich Hassiepen / Wassim Abdulrahman / Radosav S Pantelic / Syota Matsumoto / Kaoru Sugasawa / Henning Stahlberg / Nicolas H Thomä /
PubMed AbstractThe cullin-RING ubiquitin E3 ligase (CRL) family comprises over 200 members in humans. The COP9 signalosome complex (CSN) regulates CRLs by removing their ubiquitin-like activator NEDD8. The CUL4A- ...The cullin-RING ubiquitin E3 ligase (CRL) family comprises over 200 members in humans. The COP9 signalosome complex (CSN) regulates CRLs by removing their ubiquitin-like activator NEDD8. The CUL4A-RBX1-DDB1-DDB2 complex (CRL4A(DDB2)) monitors the genome for ultraviolet-light-induced DNA damage. CRL4A(DBB2) is inactive in the absence of damaged DNA and requires CSN to regulate the repair process. The structural basis of CSN binding to CRL4A(DDB2) and the principles of CSN activation are poorly understood. Here we present cryo-electron microscopy structures for CSN in complex with neddylated CRL4A ligases to 6.4 Å resolution. The CSN conformers defined by cryo-electron microscopy and a novel apo-CSN crystal structure indicate an induced-fit mechanism that drives CSN activation by neddylated CRLs. We find that CSN and a substrate cannot bind simultaneously to CRL4A, favouring a deneddylated, inactive state for substrate-free CRL4 complexes. These architectural and regulatory principles appear conserved across CRL families, allowing global regulation by CSN.
External linksNature / PubMed:27029275
MethodsEM (single particle) / X-ray diffraction
Resolution5.5 - 27.0 Å
Structure data

EMDB-3313:
Cryo-EM structure of CSN-N8-CRL4A at 6.7 A resolution
Method: EM (single particle) / Resolution: 6.7 Å

EMDB-3314:
Rigid part of the CSN-N8-CRL4A cryo-EM structure at 6.4 A resolution
Method: EM (single particle) / Resolution: 6.4 Å

EMDB-3315:
Cryo-EM structure of CSN-N8-CRL4A at 8.8 A resolution
Method: EM (single particle) / Resolution: 8.8 Å

EMDB-3316:
Cryo-EM structure of CSN-N8-CRL4ADDB2 at 8.3 A resolution
Method: EM (single particle) / Resolution: 8.3 Å

EMDB-3317:
Negative stain EM structure of CSN-N8-CRL3 at 27 A resolution
Method: EM (single particle) / Resolution: 27.0 Å

PDB-4wsn:
Crystal structure of the COP9 signalosome, a P1 crystal form
Method: X-RAY DIFFRACTION / Resolution: 5.5 Å

Chemicals

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • Danio rerio (zebrafish)
KeywordsSIGNALING PROTEIN / hydrolase

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