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TitleNanobody-mediated resistance to Grapevine fanleaf virus in plants.
Journal, issue, pagesPlant Biotechnol J, Vol. 16, Issue 2, Page 660-671, Year 2018
Publish dateOct 6, 2017
AuthorsCaroline Hemmer / Samia Djennane / Léa Ackerer / Kamal Hleibieh / Aurélie Marmonier / Sophie Gersch / Shahinez Garcia / Emmanuelle Vigne / Véronique Komar / Mireille Perrin / Claude Gertz / Lorène Belval / François Berthold / Baptiste Monsion / Corinne Schmitt-Keichinger / Olivier Lemaire / Bernard Lorber / Carlos Gutiérrez / Serge Muyldermans / Gérard Demangeat / Christophe Ritzenthaler /
PubMed AbstractSince their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as ...Since their discovery, single-domain antigen-binding fragments of camelid-derived heavy-chain-only antibodies, also known as nanobodies (Nbs), have proven to be of outstanding interest as therapeutics against human diseases and pathogens including viruses, but their use against phytopathogens remains limited. Many plant viruses including Grapevine fanleaf virus (GFLV), a nematode-transmitted icosahedral virus and causal agent of fanleaf degenerative disease, have worldwide distribution and huge burden on crop yields representing billions of US dollars of losses annually, yet solutions to combat these viruses are often limited or inefficient. Here, we identified a Nb specific to GFLV that confers strong resistance to GFLV upon stable expression in the model plant Nicotiana benthamiana and also in grapevine rootstock, the natural host of the virus. We showed that resistance was effective against a broad range of GFLV isolates independently of the inoculation method including upon nematode transmission but not against its close relative, Arabis mosaic virus. We also demonstrated that virus neutralization occurs at an early step of the virus life cycle, prior to cell-to-cell movement. Our findings will not only be instrumental to confer resistance to GFLV in grapevine, but more generally they pave the way for the generation of novel antiviral strategies in plants based on Nbs.
External linksPlant Biotechnol J / PubMed:28796912 / PubMed Central
MethodsEM (single particle)
Resolution2.8 Å
Structure data

EMDB-3246:
Electron cryo-microscopy of Grapevine Fanleaf Virus complex with Nanobody
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • synthetic construct (others)

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