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TitleStructure of SARS-CoV-2 membrane protein essential for virus assembly.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4399, Year 2022
Publish dateAug 5, 2022
AuthorsZhikuan Zhang / Norimichi Nomura / Yukiko Muramoto / Toru Ekimoto / Tomoko Uemura / Kehong Liu / Moeko Yui / Nozomu Kono / Junken Aoki / Mitsunori Ikeguchi / Takeshi Noda / So Iwata / Umeharu Ohto / Toshiyuki Shimizu /
PubMed AbstractThe coronavirus membrane protein (M) is the most abundant viral structural protein and plays a central role in virus assembly and morphogenesis. However, the process of M protein-driven virus ...The coronavirus membrane protein (M) is the most abundant viral structural protein and plays a central role in virus assembly and morphogenesis. However, the process of M protein-driven virus assembly are largely unknown. Here, we report the cryo-electron microscopy structure of the SARS-CoV-2 M protein in two different conformations. M protein forms a mushroom-shaped dimer, composed of two transmembrane domain-swapped three-helix bundles and two intravirion domains. M protein further assembles into higher-order oligomers. A highly conserved hinge region is key for conformational changes. The M protein dimer is unexpectedly similar to SARS-CoV-2 ORF3a, a viral ion channel. Moreover, the interaction analyses of M protein with nucleocapsid protein (N) and RNA suggest that the M protein mediates the concerted recruitment of these components through the positively charged intravirion domain. Our data shed light on the M protein-driven virus assembly mechanism and provide a structural basis for therapeutic intervention targeting M protein.
External linksNat Commun / PubMed:35931673 / PubMed Central
MethodsEM (single particle)
Resolution2.7 - 6.2 Å
Structure data

EMDB-31976: Cryo-EM reconstruction of SARS-CoV-2 M protein dimer in LMNG/CHS micelle
Method: EM (single particle) / Resolution: 6.2 Å

31977

7vgr

EMDB-31977, PDB-7vgr:
SARS-CoV-2 M protein dimer (long form) in complex with YN7756_1 Fab
Method: EM (single particle) / Resolution: 2.7 Å

31978

7vgs

EMDB-31978, PDB-7vgs:
SARS-CoV-2 M protein dimer (short form) in complex with YN7717_9 Fab
Method: EM (single particle) / Resolution: 2.8 Å

Source
  • severe acute respiratory syndrome coronavirus 2
  • mus musculus (house mouse)
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / SARS-CoV-2 / M protein / viral structural protein / virus assembly / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex

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