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TitleFunctional Dynamics Revealed by the Structure of the SufBCD Complex, a Novel ATP-binding Cassette (ABC) Protein That Serves as a Scaffold for Iron-Sulfur Cluster Biogenesis.
Journal, issue, pagesJ Biol Chem, Vol. 290, Issue 50, Page 29717-29731, Year 2015
Publish dateDec 11, 2015
AuthorsKei Hirabayashi / Eiki Yuda / Naoyuki Tanaka / Sumie Katayama / Kenji Iwasaki / Takashi Matsumoto / Genji Kurisu / F Wayne Outten / Keiichi Fukuyama / Yasuhiro Takahashi / Kei Wada /
PubMed AbstractATP-binding cassette (ABC)-type ATPases are chemomechanical engines involved in diverse biological pathways. Recent genomic information reveals that ABC ATPase domains/subunits act not only in ABC ...ATP-binding cassette (ABC)-type ATPases are chemomechanical engines involved in diverse biological pathways. Recent genomic information reveals that ABC ATPase domains/subunits act not only in ABC transporters and structural maintenance of chromosome proteins, but also in iron-sulfur (Fe-S) cluster biogenesis. A novel type of ABC protein, the SufBCD complex, functions in the biosynthesis of nascent Fe-S clusters in almost all Eubacteria and Archaea, as well as eukaryotic chloroplasts. In this study, we determined the first crystal structure of the Escherichia coli SufBCD complex, which exhibits the common architecture of ABC proteins: two ABC ATPase components (SufC) with function-specific components (SufB-SufD protomers). Biochemical and physiological analyses based on this structure provided critical insights into Fe-S cluster assembly and revealed a dynamic conformational change driven by ABC ATPase activity. We propose a molecular mechanism for the biogenesis of the Fe-S cluster in the SufBCD complex.
External linksJ Biol Chem / PubMed:26472926 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.957 - 30.0 Å
Structure data

EMDB-3163:
Single-particle reconstruction of negatively stained SufBCD
Method: EM (single particle) / Resolution: 30.0 Å

PDB-5awf:
Crystal structure of SufB-SufC-SufD complex from Escherichia coli
Method: X-RAY DIFFRACTION / Resolution: 2.957 Å

PDB-5awg:
Crystal structure of Hg-bound SufB-SufC-SufD complex from Escherichia coli
Method: X-RAY DIFFRACTION / Resolution: 4.278 Å

Chemicals

ChemComp-HG:
Unknown entry / Mercury (element)

Source
  • Escherichia coli K-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsTRANSPORT PROTEIN/PROTEIN BINDING / Iron-Sulfur Clusters / Iron-Sulfur Proteins / ABC proteins / ABC ATPase / TRANSPORT PROTEIN-PROTEIN BINDING complex

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