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TitleInsertion of the Biogenesis Factor Rei1 Probes the Ribosomal Tunnel during 60S Maturation.
Journal, issue, pagesCell, Vol. 164, Issue 1-2, Page 91-9102, Year 2016
Publish dateJan 14, 2016
AuthorsBasil Johannes Greber / Stefan Gerhardy / Alexander Leitner / Marc Leibundgut / Michèle Salem / Daniel Boehringer / Nicolas Leulliot / Ruedi Aebersold / Vikram Govind Panse / Nenad Ban /
PubMed AbstractEukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, ...Eukaryotic ribosome biogenesis depends on several hundred assembly factors to produce functional 40S and 60S ribosomal subunits. The final phase of 60S subunit biogenesis is cytoplasmic maturation, which includes the proofreading of functional centers of the 60S subunit and the release of several ribosome biogenesis factors. We report the cryo-electron microscopy (cryo-EM) structure of the yeast 60S subunit in complex with the biogenesis factors Rei1, Arx1, and Alb1 at 3.4 Å resolution. In addition to the network of interactions formed by Alb1, the structure reveals a mechanism for ensuring the integrity of the ribosomal polypeptide exit tunnel. Arx1 probes the entire set of inner-ring proteins surrounding the tunnel exit, and the C terminus of Rei1 is deeply inserted into the ribosomal tunnel, where it forms specific contacts along almost its entire length. We provide genetic and biochemical evidence that failure to insert the C terminus of Rei1 precludes subsequent steps of 60S maturation.
External linksCell / PubMed:26709046
MethodsEM (single particle)
Resolution3.4 - 3.91 Å
Structure data

3151

5apo

EMDB-3151: Cryo-EM Structure of the 60S-Arx1-Alb1-Rei1 Complex
PDB-5apo: Structure of the yeast 60S ribosomal subunit in complex with Arx1, Alb1 and C-terminally tagged Rei1
Method: EM (single particle) / Resolution: 3.4 Å

3152

5apn

EMDB-3152: Cryo-EM structure of the 60S-Arx1-Alb1-Rei1 complex (Rei1 N-terminal His6-tag)
PDB-5apn: Structure of the yeast 60S ribosomal subunit in complex with Arx1, Alb1 and N-terminally tagged Rei1
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-3153:
Cryo-EM structure of the 60S-Arx1-Rei1 complex at near-atomic resolution
Method: EM (single particle) / Resolution: 3.7 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsRIBOSOME / EUKARYOTIC RIBOSOME BIOGENESIS / RIBOSOME MATURATION / RIBOSOME BIOGENESIS FACTOR / 60S RIBOSOMAL SUBUNIT / REI1 / ARX1 / ALB1 / CRYO-EM / RIBOSOMAL POLYPEPTIDE EXIT TUNNEL PROOFREADING

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