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TitleStructural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.
Journal, issue, pagesSci Adv, Vol. 8, Issue 4, Page eabi5603, Year 2022
Publish dateJan 28, 2022
AuthorsChenchen Mi / Li Zhang / Guoqiang Huang / Guangcan Shao / Fan Yang / Xin You / Meng-Qiu Dong / Shan Sun / Sen-Fang Sui /
PubMed AbstractTransport protein particle (TRAPP) complexes belong to the multiprotein tethering complex and exist in three forms-core TRAPP/TRAPPI, TRAPPII, and TRAPPIII. TRAPPII activates GTPase Ypt31/Ypt32 as ...Transport protein particle (TRAPP) complexes belong to the multiprotein tethering complex and exist in three forms-core TRAPP/TRAPPI, TRAPPII, and TRAPPIII. TRAPPII activates GTPase Ypt31/Ypt32 as the guanine nucleotide exchange factor in the trans-Golgi network to determine the maturation of Golgi cisternae into post-Golgi carriers in yeast. Here, we present cryo-EM structures of yeast TRAPPII in apo and Ypt32-bound states. All the structures show a dimeric architecture assembled by two triangle-shaped monomers, while the monomer in the apo state exhibits both open and closed conformations, and the monomer in the Ypt32-bound form only captures the closed conformation. Located in the interior of the monomer, Ypt32 binds with both core TRAPP/TRAPPI and Trs120 via its nucleotide-binding domain and binds with Trs31 via its hypervariable domain. Combined with functional analysis, the structures provide insights into the assembly of TRAPPII and the mechanism of the specific activation of Ypt31/Ypt32 by TRAPPII.
External linksSci Adv / PubMed:35080977 / PubMed Central
MethodsEM (single particle)
Resolution3.71 - 6.54 Å
Structure data

30954

7e2c

EMDB-30954, PDB-7e2c:
Monomer of TRAPPII (open)
Method: EM (single particle) / Resolution: 4.18 Å

30955

7e2d

EMDB-30955, PDB-7e2d:
Monomer of TRAPPII (Closed)
Method: EM (single particle) / Resolution: 3.71 Å

31021

7e8s

EMDB-31021, PDB-7e8s:
Intact TRAPPII (state I).
Method: EM (single particle) / Resolution: 4.36 Å

31022

7e8t

EMDB-31022, PDB-7e8t:
Monomer of Ypt32-TRAPPII
Method: EM (single particle) / Resolution: 3.8 Å

31027

7e93

EMDB-31027, PDB-7e93:
Intact TRAPPII (state III).
Method: EM (single particle) / Resolution: 6.54 Å

31028

7e94

EMDB-31028, PDB-7e94:
Intact TRAPPII (State II)
Method: EM (single particle) / Resolution: 4.67 Å

31038

7ea3

EMDB-31038, PDB-7ea3:
Intact Ypt32-TRAPPII (dimer).
Method: EM (single particle) / Resolution: 4.31 Å

Source
  • Saccharomyces cerevisiae (brewer's yeast)
  • saccharomyces cerevisiae (strain atcc 204508 / s288c) (yeast)
KeywordsPROTEIN TRANSPORT / protein transport TRAPPII open / Monomer of TRAPPII close state. / TRANSPORT PROTEIN / Complex

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