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TitleStructural characterization of the Plasmodium falciparum lactate transporter PfFNT alone and in complex with antimalarial compound MMV007839 reveals its inhibition mechanism.
Journal, issue, pagesPLoS Biol, Vol. 19, Issue 9, Page e3001386, Year 2021
Publish dateSep 9, 2021
AuthorsXi Peng / Nan Wang / Angqi Zhu / Hanwen Xu / Jialu Li / Yanxia Zhou / Chen Wang / Qingjie Xiao / Li Guo / Fei Liu / Zhi-Jun Jia / Huaichuan Duan / Jianping Hu / Weidan Yuan / Jia Geng / Chuangye Yan / Xin Jiang / Dong Deng /
PubMed AbstractPlasmodium falciparum, the deadliest causal agent of malaria, caused more than half of the 229 million malaria cases worldwide in 2019. The emergence and spreading of frontline drug-resistant ...Plasmodium falciparum, the deadliest causal agent of malaria, caused more than half of the 229 million malaria cases worldwide in 2019. The emergence and spreading of frontline drug-resistant Plasmodium strains are challenging to overcome in the battle against malaria and raise urgent demands for novel antimalarial agents. The P. falciparum formate-nitrite transporter (PfFNT) is a potential drug target due to its housekeeping role in lactate efflux during the intraerythrocytic stage. Targeting PfFNT, MMV007839 was identified as a lead compound that kills parasites at submicromolar concentrations. Here, we present 2 cryogenic-electron microscopy (cryo-EM) structures of PfFNT, one with the protein in its apo form and one with it in complex with MMV007839, both at 2.3 Å resolution. Benefiting from the high-resolution structures, our study provides the molecular basis for both the lactate transport of PfFNT and the inhibition mechanism of MMV007839, which facilitates further antimalarial drug design.
External linksPLoS Biol / PubMed:34499638 / PubMed Central
MethodsEM (single particle)
Resolution2.29 Å
Structure data

30952

7e26

EMDB-30952, PDB-7e26:
Structure of PfFNT in apo state
Method: EM (single particle) / Resolution: 2.29 Å

30953

7e27

EMDB-30953, PDB-7e27:
Structure of PfFNT in complex with MMV007839
Method: EM (single particle) / Resolution: 2.29 Å

Chemicals

ChemComp-HOH:
WATER / Water

ChemComp-HV6:
(Z)-4,4,5,5,5-pentakis(fluoranyl)-1-(4-methoxy-2-oxidanyl-phenyl)-3-oxidanyl-pent-2-en-1-one

Source
  • plasmodium falciparum 3d7 (eukaryote)
KeywordsTRANSPORT PROTEIN / Lactate / transporter / lactate transporter

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