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Title | An infectivity-enhancing site on the SARS-CoV-2 spike protein targeted by antibodies. |
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Journal, issue, pages | Cell, Vol. 184, Issue 13, Page 3452-3466.e18, Year 2021 |
Publish date | Jun 24, 2021 |
Authors | Yafei Liu / Wai Tuck Soh / Jun-Ichi Kishikawa / Mika Hirose / Emi E Nakayama / Songling Li / Miwa Sasai / Tatsuya Suzuki / Asa Tada / Akemi Arakawa / Sumiko Matsuoka / Kanako Akamatsu / Makoto Matsuda / Chikako Ono / Shiho Torii / Kazuki Kishida / Hui Jin / Wataru Nakai / Noriko Arase / Atsushi Nakagawa / Maki Matsumoto / Yukoh Nakazaki / Yasuhiro Shindo / Masako Kohyama / Keisuke Tomii / Koichiro Ohmura / Shiro Ohshima / Toru Okamoto / Masahiro Yamamoto / Hironori Nakagami / Yoshiharu Matsuura / Atsushi Nakagawa / Takayuki Kato / Masato Okada / Daron M Standley / Tatsuo Shioda / Hisashi Arase / |
PubMed Abstract | Antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein prevent SARS-CoV-2 infection. However, the effects of antibodies against other spike protein domains are largely ...Antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein prevent SARS-CoV-2 infection. However, the effects of antibodies against other spike protein domains are largely unknown. Here, we screened a series of anti-spike monoclonal antibodies from coronavirus disease 2019 (COVID-19) patients and found that some of antibodies against the N-terminal domain (NTD) induced the open conformation of RBD and thus enhanced the binding capacity of the spike protein to ACE2 and infectivity of SARS-CoV-2. Mutational analysis revealed that all of the infectivity-enhancing antibodies recognized a specific site on the NTD. Structural analysis demonstrated that all infectivity-enhancing antibodies bound to NTD in a similar manner. The antibodies against this infectivity-enhancing site were detected at high levels in severe patients. Moreover, we identified antibodies against the infectivity-enhancing site in uninfected donors, albeit at a lower frequency. These findings demonstrate that not only neutralizing antibodies but also enhancing antibodies are produced during SARS-CoV-2 infection. |
External links | Cell / PubMed:34139176 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.45 - 6.08 Å |
Structure data | EMDB-30915: Apo spike protein of SARS-CoV2 EMDB-30916: EMDB-30917: EMDB-30918: SARS-CoV2 spike protein with Fab fragment of enhancing antibody (8D2)-2 EMDB-30919: EMDB-30920: EMDB-30921: SARS-CoV2 spike protein with Fab fragment of enhancing antibody (2940)-1 EMDB-30922: |
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Keywords | VIRAL PROTEIN / SARS-CoV2 / spike protein / Fab / enhancing antibody |