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TitleExon and protein positioning in a pre-catalytic group II intron RNP primed for splicing.
Journal, issue, pagesNucleic Acids Res, Vol. 48, Issue 19, Page 11185-11198, Year 2020
Publish dateNov 4, 2020
AuthorsNan Liu / Xiaolong Dong / Cuixia Hu / Jianwei Zeng / Jiawei Wang / Jia Wang / Hong-Wei Wang / Marlene Belfort /
PubMed AbstractGroup II introns are the putative progenitors of nuclear spliceosomal introns and use the same two-step splicing pathway. In the cell, the intron RNA forms a ribonucleoprotein (RNP) complex with the ...Group II introns are the putative progenitors of nuclear spliceosomal introns and use the same two-step splicing pathway. In the cell, the intron RNA forms a ribonucleoprotein (RNP) complex with the intron-encoded protein (IEP), which is essential for splicing. Although structures of spliced group II intron RNAs and RNP complexes have been characterized, structural insights into the splicing process remain enigmatic due to lack of pre-catalytic structural models. Here, we report two cryo-EM structures of endogenously produced group II intron RNPs trapped in their pre-catalytic state. Comparison of the catalytically activated precursor RNP to its previously reported spliced counterpart allowed identification of key structural rearrangements accompanying splicing, including a remodeled active site and engagement of the exons. Importantly, altered RNA-protein interactions were observed upon splicing among the RNP complexes. Furthermore, analysis of the catalytically inert precursor RNP demonstrated the structural impact of the formation of the active site on RNP architecture. Taken together, our results not only fill a gap in understanding the structural basis of IEP-assisted group II intron splicing, but also provide parallels to evolutionarily related spliceosomal splicing.
External linksNucleic Acids Res / PubMed:33021674 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 8.4 Å
Structure data

30532

7d0f

EMDB-30532, PDB-7d0f:
cryo-EM structure of a pre-catalytic group II intron RNP
Method: EM (single particle) / Resolution: 5.0 Å

30533

7d0g

EMDB-30533, PDB-7d0g:
Cryo-EM structure of a pre-catalytic group II intron
Method: EM (single particle) / Resolution: 5.0 Å

EMDB-30540:
cryo-EM structure of a pre-catalytic group II intron complex
Method: EM (single particle) / Resolution: 8.4 Å

EMDB-30541:
cryo-EM structure of a pre-catalytic group II intron complex
Method: EM (single particle) / Resolution: 5.1 Å

PDB-7d1a:
cryo-EM structure of a group II intron RNP complexed with its reverse transcriptase
Method: ELECTRON MICROSCOPY / Resolution: 3.8 Å

Source
  • lactococcus lactis subsp. cremoris (lactic acid bacteria)
KeywordsTRANSFERASE/RNA / catalytic RNA / RNA-protein interactions / group II intron / TRANSFERASE-RNA complex / RNA-protein complex / pre-catalytic

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