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TitleStructure and assembly of double-headed Sendai virus nucleocapsids.
Journal, issue, pagesCommun Biol, Vol. 4, Issue 1, Page 494, Year 2021
Publish dateApr 22, 2021
AuthorsNa Zhang / Hong Shan / Mingdong Liu / Tianhao Li / Rui Luo / Liuyan Yang / Lei Qi / Xiaofeng Chu / Xin Su / Rui Wang / Yunhui Liu / Wenzhi Sun / Qing-Tao Shen /
PubMed AbstractParamyxoviruses, including the mumps virus, measles virus, Nipah virus and Sendai virus (SeV), have non-segmented single-stranded negative-sense RNA genomes which are encapsidated by nucleoproteins ...Paramyxoviruses, including the mumps virus, measles virus, Nipah virus and Sendai virus (SeV), have non-segmented single-stranded negative-sense RNA genomes which are encapsidated by nucleoproteins into helical nucleocapsids. Here, we reported a double-headed SeV nucleocapsid assembled in a tail-to-tail manner, and resolved its helical stems and clam-shaped joint at the respective resolutions of 2.9 and 3.9 Å, via cryo-electron microscopy. Our structures offer important insights into the mechanism of the helical polymerization, in particular via an unnoticed exchange of a N-terminal hole formed by three loops of nucleoproteins, and unveil the clam-shaped joint in a hyper-closed state for nucleocapsid dimerization. Direct visualization of the loop from the disordered C-terminal tail provides structural evidence that C-terminal tail is correlated to the curvature of nucleocapsid and links nucleocapsid condensation and genome replication and transcription with different assembly forms.
External linksCommun Biol / PubMed:33888861 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.9 - 4.61 Å
Structure data

EMDB-30064:
Clam-shaped joint of double-headed nucleocapsid of Sendai Virus
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-30065:
helical stem of double-headed nucleocapsid of Sendai virus in 4.6 angstrom resolution
Method: EM (helical sym.) / Resolution: 4.61 Å

EMDB-30066:
Helical stem of double-headed nucleocapsid of Sendai virus in 4.1 angstrom resolution
Method: EM (helical sym.) / Resolution: 4.09 Å

30129

6m7d

EMDB-30129: Helical stem of the cleaved double-headed nucleocapsids of sendai virus
PDB-6m7d: Structure of ncleoprotein of sendai virus
Method: EM (helical sym.) / Resolution: 2.9 Å

EMDB-30133:
Helix stem of the nucleocapsid of sendaivirus treated by trypsin
Method: EM (helical sym.) / Resolution: 3.81 Å

Source
  • Murine respirovirus
  • sendai virus (strain ohita)
  • synthetic construct (others)
KeywordsNUCLEAR PROTEIN/RNA / nucleocapsid / Sendai virus / NUCLEAR PROTEIN / NUCLEAR PROTEIN-RNA complex

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