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TitleThe ϕPA3 phage nucleus is enclosed by a self-assembling 2D crystalline lattice.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 927, Year 2023
Publish dateFeb 18, 2023
AuthorsEliza S Nieweglowska / Axel F Brilot / Melissa Méndez-Moran / Claire Kokontis / Minkyung Baek / Junrui Li / Yifan Cheng / David Baker / Joseph Bondy-Denomy / David A Agard /
PubMed AbstractTo protect themselves from host attack, numerous jumbo bacteriophages establish a phage nucleus-a micron-scale, proteinaceous structure encompassing the replicating phage DNA. Bacteriophage and host ...To protect themselves from host attack, numerous jumbo bacteriophages establish a phage nucleus-a micron-scale, proteinaceous structure encompassing the replicating phage DNA. Bacteriophage and host proteins associated with replication and transcription are concentrated inside the phage nucleus while other phage and host proteins are excluded, including CRISPR-Cas and restriction endonuclease host defense systems. Here, we show that nucleus fragments isolated from ϕPA3 infected Pseudomonas aeruginosa form a 2-dimensional lattice, having p2 or p4 symmetry. We further demonstrate that recombinantly purified primary Phage Nuclear Enclosure (PhuN) protein spontaneously assembles into similar 2D sheets with p2 and p4 symmetry. We resolve the dominant p2 symmetric state to 3.9 Å by cryo-EM. Our structure reveals a two-domain core, organized into quasi-symmetric tetramers. Flexible loops and termini mediate adaptable inter-tetramer contacts that drive subunit assembly into a lattice and enable the adoption of different symmetric states. While the interfaces between subunits are mostly well packed, two are open, forming channels that likely have functional implications for the transport of proteins, mRNA, and small molecules.
External linksNat Commun / PubMed:36807264 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 4.21 Å
Structure data

29310

8fne

EMDB-29310: Deconvolved phiPA3 PhuN Tetramer, p2
PDB-8fne: phiPA3 PhuN Tetramer, p2
Method: EM (single particle) / Resolution: 3.9 Å

29451

8fv5

EMDB-29451: Tracing p2 phiPA3 PhuN Tetramer Interfaces
PDB-8fv5: Representation of 16-mer phiPA3 PhuN Lattice, p2
Method: EM (single particle) / Resolution: 4.21 Å

EMDB-29550: phiPA3 PhuN Tetramer, p2
Method: EM (single particle) / Resolution: 3.9 Å

Source
  • pseudomonas phage phipa3 (virus)
  • escherichia coli k-12 (bacteria)
  • escherichia coli (strain k12) (bacteria)
KeywordsVIRAL PROTEIN / phiPA3 protein / shell protein / PhuN / phage nucleus

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