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TitleCryo-EM structure of the Agrobacterium tumefaciens T4SS-associated T-pilus reveals stoichiometric protein-phospholipid assembly.
Journal, issue, pagesStructure, Vol. 31, Issue 4, Page 385-394.e4, Year 2023
Publish dateApr 6, 2023
AuthorsStefan Kreida / Akihiro Narita / Matthew D Johnson / Elitza I Tocheva / Anath Das / Debnath Ghosal / Grant J Jensen /
PubMed AbstractAgrobacterium tumefaciens causes crown gall disease in plants by the horizontal transfer of oncogenic DNA. The conjugation is mediated by the VirB/D4 type 4 secretion system (T4SS) that assembles an ...Agrobacterium tumefaciens causes crown gall disease in plants by the horizontal transfer of oncogenic DNA. The conjugation is mediated by the VirB/D4 type 4 secretion system (T4SS) that assembles an extracellular filament, the T-pilus, and is involved in mating pair formation between A. tumefaciens and the recipient plant cell. Here, we present a 3 Å cryoelectron microscopy (cryo-EM) structure of the T-pilus solved by helical reconstruction. Our structure reveals that the T-pilus is a stoichiometric assembly of the VirB2 major pilin and phosphatidylglycerol (PG) phospholipid with 5-start helical symmetry. We show that PG head groups and the positively charged Arg 91 residues of VirB2 protomers form extensive electrostatic interactions in the lumen of the T-pilus. Mutagenesis of Arg 91 abolished pilus formation. While our T-pilus structure is architecturally similar to previously published conjugative pili structures, the T-pilus lumen is narrower and positively charged, raising questions of whether the T-pilus is a conduit for ssDNA transfer.
External linksStructure / PubMed:36870333 / PubMed Central
MethodsEM (helical sym.)
Resolution3.0 Å
Structure data

28957

8fai

EMDB-28957, PDB-8fai:
Cryo-EM structure of the Agrobacterium T-pilus
Method: EM (helical sym.) / Resolution: 3.0 Å

Chemicals


ChemComp, No image

ChemComp-XL0:
Unknown entry

Source
  • agrobacterium tumefaciens (bacteria)
KeywordsPROTEIN FIBRIL / VirB/D4 T4SS / Bacterial conjugation / T-pilus / helical filament

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