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TitleCryo-EM structures of anti-malarial antibody L9 with circumsporozoite protein reveal trimeric L9 association and complete 27-residue epitope.
Journal, issue, pagesStructure, Vol. 31, Issue 4, Page 480-491.e4, Year 2023
Publish dateApr 6, 2023
AuthorsPrabhanshu Tripathi / Michael F Bender / Haotian Lei / Lais Da Silva Pereira / Chen-Hsiang Shen / Brian Bonilla / Marlon Dillon / Li Ou / Marie Pancera / Lawrence T Wang / Baoshan Zhang / Facundo D Batista / Azza H Idris / Robert A Seder / Peter D Kwong /
PubMed AbstractMonoclonal antibody L9 recognizes the Plasmodium falciparum circumsporozoite protein (PfCSP) and is highly protective following controlled human malaria challenge. To gain insight into its function, ...Monoclonal antibody L9 recognizes the Plasmodium falciparum circumsporozoite protein (PfCSP) and is highly protective following controlled human malaria challenge. To gain insight into its function, we determined cryoelectron microscopy (cryo-EM) structures of L9 in complex with full-length PfCSP and assessed how this recognition influenced protection by wild-type and mutant L9s. Cryo-EM reconstructions at 3.6- and 3.7-Å resolution revealed L9 to recognize PfCSP as an atypical trimer. Each of the three L9s in the trimer directly recognized an Asn-Pro-Asn-Val (NPNV) tetrapeptide on PfCSP and interacted homotypically to facilitate L9-trimer assembly. We analyzed peptides containing different repeat tetrapeptides for binding to wild-type and mutant L9s to delineate epitope and homotypic components of L9 recognition; we found both components necessary for potent malaria protection. Last, we found the 27-residue stretch recognized by L9 to be highly conserved in P. falciparum isolates, suggesting the newly revealed complete L9 epitope to be an attractive vaccine target.
External linksStructure / PubMed:36931276 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 3.7 Å
Structure data

28192

8ek1

EMDB-28192, PDB-8ek1:
Cryo-EM structure of a potent anti-malarial antibody L9 in complex with Plasmodium falciparum circumsporozoite protein (PfCSP)(dominant class)
Method: EM (single particle) / Resolution: 3.6 Å

28196

8eka

EMDB-28196, PDB-8eka:
Cryo-EM structure of a potent anti-malarial antibody L9 in complex with Plasmodium falciparum circumsporozoite protein (PfCSP)(class 2)
Method: EM (single particle) / Resolution: 3.7 Å

Source
  • homo sapiens (human)
  • plasmodium falciparum 3d7 (eukaryote)
KeywordsIMMUNE SYSTEM / Anti-Malarial / PfCSP

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