Alexander G Myasnikov / Zhanna A Afonina / Jean-François Ménétret / Vladimir A Shirokov / Alexander S Spirin / Bruno P Klaholz /
PubMed Abstract
During protein synthesis, several ribosomes bind to a single messenger RNA (mRNA) forming large macromolecular assemblies called polyribosomes. Here we report the detailed molecular structure of a ...During protein synthesis, several ribosomes bind to a single messenger RNA (mRNA) forming large macromolecular assemblies called polyribosomes. Here we report the detailed molecular structure of a 100 MDa eukaryotic poly-ribosome complex derived from cryo electron tomography, sub-tomogram averaging and pseudo-atomic modelling by crystal structure fitting. The structure allowed the visualization of the three functional parts of the polysome assembly, the central core region that forms a rather compact left-handed supra-molecular helix, and the more open regions that harbour the initiation and termination sites at either ends. The helical region forms a continuous mRNA channel where the mRNA strand bridges neighbouring exit and entry sites of the ribosomes and prevents mRNA looping between ribosomes. This structure provides unprecedented insights into protein- and RNA-mediated inter-ribosome contacts that involve conserved sites through 40S subunits and long protruding RNA expansion segments, suggesting a role in stabilizing the overall polyribosomal assembly.
EMDB-2790: The molecular structure of the left-handed supra- molecular helix of eukaryotic polyribosomes PDB-4v3p: The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes Method: EM (subtomogram averaging) / Resolution: 34.0 Å
Source
triticum aestivum (bread wheat)
Keywords
RIBOSOME
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