EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Multistep loading of a DNA sliding clamp onto DNA by replication factor C.
ジャーナル・号・ページ	Elife, Vol. 11, Year 2022
掲載日	2022年8月8日
著者	Marina Schrecker / Juan C Castaneda / Sujan Devbhandari / Charanya Kumar / Dirk Remus / Richard K Hite /
PubMed 要旨	The DNA sliding clamp proliferating cell nuclear antigen (PCNA) is an essential co-factor for many eukaryotic DNA metabolic enzymes. PCNA is loaded around DNA by the ATP-dependent clamp loader ...The DNA sliding clamp proliferating cell nuclear antigen (PCNA) is an essential co-factor for many eukaryotic DNA metabolic enzymes. PCNA is loaded around DNA by the ATP-dependent clamp loader replication factor C (RFC), which acts at single-stranded (ss)/double-stranded DNA (dsDNA) junctions harboring a recessed 3' end (3' ss/dsDNA junctions) and at DNA nicks. To illuminate the loading mechanism we have investigated the structure of RFC:PCNA bound to ATPγS and 3' ss/dsDNA junctions or nicked DNA using cryogenic electron microscopy. Unexpectedly, we observe open and closed PCNA conformations in the RFC:PCNA:DNA complex, revealing that PCNA can adopt an open, planar conformation that allows direct insertion of dsDNA, and raising the question of whether PCNA ring closure is mechanistically coupled to ATP hydrolysis. By resolving multiple DNA-bound states of RFC:PCNA we observe that partial melting facilitates lateral insertion into the central channel formed by RFC:PCNA. We also resolve the Rfc1 N-terminal domain and demonstrate that its single BRCT domain participates in coordinating DNA prior to insertion into the central RFC channel, which promotes PCNA loading on the lagging strand of replication forks in vitro. Combined, our data suggest a comprehensive and fundamentally revised model for the RFC-catalyzed loading of PCNA onto DNA.
リンク	Elife / PubMed:35939393 / PubMed Central
手法	EM (単粒子)
解像度	2.1 - 2.92 Å
構造データ	27662 8dqw EMDB-27662, PDB-8dqw: Open state of Rad24-RFC:9-1-1 bound to a 5' ss/dsDNA junction 手法: EM (単粒子) / 解像度: 2.1 Å 27663 8dqx EMDB-27663, PDB-8dqx: Open state of RFC:PCNA bound to a 3' ss/dsDNA junction 手法: EM (単粒子) / 解像度: 2.1 Å 27666 8dqz EMDB-27666, PDB-8dqz: Intermediate state of RFC:PCNA bound to a 3' ss/dsDNA junction 手法: EM (単粒子) / 解像度: 2.92 Å 27667 8dr0 EMDB-27667, PDB-8dr0: Closed state of RFC:PCNA bound to a 3' ss/dsDNA junction 手法: EM (単粒子) / 解像度: 2.42 Å 27668 8dr1 EMDB-27668, PDB-8dr1: Consensus closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) 手法: EM (単粒子) / 解像度: 2.14 Å 27669 8dr3 EMDB-27669, PDB-8dr3: Closed state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) with NTD 手法: EM (単粒子) / 解像度: 2.2 Å 27670 8dr4 EMDB-27670, PDB-8dr4: Open state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) without NTD 手法: EM (単粒子) / 解像度: 2.45 Å 27671 8dr5 EMDB-27671, PDB-8dr5: Open state of RFC:PCNA bound to a 3' ss/dsDNA junction (DNA2) with NTD 手法: EM (単粒子) / 解像度: 2.76 Å 27672 8dr6 EMDB-27672, PDB-8dr6: Closed state of RFC:PCNA bound to a nicked dsDNA 手法: EM (単粒子) / 解像度: 2.39 Å 27673 8dr7 EMDB-27673, PDB-8dr7: Open state of RFC:PCNA bound to a nicked dsDNA 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-HOH: WATER / 水 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子*YM / アデノシン二リン酸
由来	saccharomyces cerevisiae (パン酵母)
キーワード	REPLICATION/DNA / DNA damage checkpoint (DNA修復) / REPLICATION-DNA complex