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Structure paper

TitleStructural changes enable start codon recognition by the eukaryotic translation initiation complex.
Journal, issue, pagesCell, Vol. 159, Issue 3, Page 597-607, Year 2014
Publish dateOct 23, 2014
AuthorsTanweer Hussain / Jose L Llácer / Israel S Fernández / Antonio Munoz / Pilar Martin-Marcos / Christos G Savva / Jon R Lorsch / Alan G Hinnebusch / V Ramakrishnan /
PubMed AbstractDuring eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the ...During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon.
External linksCell / PubMed:25417110 / PubMed Central
MethodsEM (single particle)
Resolution3.75 - 4.0 Å
Structure data

2763

3j81

EMDB-2763, PDB-3j81:
CryoEM structure of a partial yeast 48S preinitiation complex
Method: EM (single particle) / Resolution: 4.0 Å

2764

3j80

EMDB-2764: CryoEM structure of the 40S-eIF1-eIF1A preinitiation complex
PDB-3j80: CryoEM structure of 40S-eIF1-eIF1A preinitiation complex
Method: EM (single particle) / Resolution: 3.75 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-MET:
METHIONINE / Methionine

Source
  • kluyveromyces lactis (yeast)
  • saccharomyces cerevisiae (brewer's yeast)
  • synthetic construct (others)
KeywordsRIBOSOME / small ribosome subunit / eukaryotic translation initiation / 48S

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