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TitleCoupled catalytic states and the role of metal coordination in Cas9
Journal, issue, pagesNat Catal, Vol. 6, Page 969-977, Year 2023
Publish dateMay 30, 2022 (structure data deposition date)
AuthorsDas A / Rai J / Roth MO / Shu Y / Medina ML / Barakat MR / Li H
External linksNat Catal / Search PubMed
MethodsEM (single particle)
Resolution2.21 - 2.88 Å
Structure data

27141

8d2k

EMDB-27141, PDB-8d2k:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 2)
Method: EM (single particle) / Resolution: 2.43 Å

27142

8d2l

EMDB-27142, PDB-8d2l:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Cleavage Intermediate 1)
Method: EM (single particle) / Resolution: 2.21 Å

27143

8d2n

EMDB-27143, PDB-8d2n:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Pre-cleavage)
Method: EM (single particle) / Resolution: 2.88 Å

27144

8d2o

EMDB-27144, PDB-8d2o:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 2)
Method: EM (single particle) / Resolution: 2.66 Å

27145

8d2p

EMDB-27145, PDB-8d2p:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Target bound)
Method: EM (single particle) / Resolution: 2.78 Å

27146

8d2q

EMDB-27146, PDB-8d2q:
Structure of Acidothermus cellulolyticus Cas9 ternary complex (Post-cleavage 1)
Method: EM (single particle) / Resolution: 2.58 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • acidothermus cellulolyticus 11b (bacteria)
  • acidothermus cellulolyticus (bacteria)
  • synthetic construct (others)
KeywordsRNA BINDING PROTEIN/DNA/RNA / Cas9 / AcCas9 / Crispr / RNA BINDING PROTEIN / RNA BINDING PROTEIN-DNA-RNA complex / Post-cleavage 2 / AceCas9 / Target bound / Post-Cleavage 1

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