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TitleCo-immunization with hemagglutinin stem immunogens elicits cross-group neutralizing antibodies and broad protection against influenza A viruses.
Journal, issue, pagesImmunity, Vol. 55, Issue 12, Page 2405-22418.e7, Year 2022
Publish dateDec 13, 2022
AuthorsSyed M Moin / Jeffrey C Boyington / Seyhan Boyoglu-Barnum / Rebecca A Gillespie / Gabriele Cerutti / Crystal Sao-Fong Cheung / Alberto Cagigi / John R Gallagher / Joshua Brand / Madhu Prabhakaran / Yaroslav Tsybovsky / Tyler Stephens / Brian E Fisher / Adrian Creanga / Sila Ataca / Reda Rawi / Kizzmekia S Corbett / Michelle C Crank / Gunilla B Karlsson Hedestam / Jason Gorman / Adrian B McDermott / Audray K Harris / Tongqing Zhou / Peter D Kwong / Lawrence Shapiro / John R Mascola / Barney S Graham / Masaru Kanekiyo /
PubMed AbstractCurrent influenza vaccines predominantly induce immunity to the hypervariable hemagglutinin (HA) head, requiring frequent vaccine reformulation. Conversely, the immunosubdominant yet conserved HA ...Current influenza vaccines predominantly induce immunity to the hypervariable hemagglutinin (HA) head, requiring frequent vaccine reformulation. Conversely, the immunosubdominant yet conserved HA stem harbors a supersite that is targeted by broadly neutralizing antibodies (bnAbs), representing a prime target for universal vaccines. Here, we showed that the co-immunization of two HA stem immunogens derived from group 1 and 2 influenza A viruses elicits cross-group protective immunity and neutralizing antibody responses in mice, ferrets, and nonhuman primates (NHPs). Immunized mice were protected from multiple group 1 and 2 viruses, and all animal models showed broad serum-neutralizing activity. A bnAb isolated from an immunized NHP broadly neutralized and protected against diverse viruses, including H5N1 and H7N9. Genetic and structural analyses revealed strong homology between macaque and human bnAbs, illustrating common biophysical constraints for acquiring cross-group specificity. Vaccine elicitation of stem-directed cross-group-protective immunity represents a step toward the development of broadly protective influenza vaccines.
External linksImmunity / PubMed:36356572 / PubMed Central
MethodsEM (single particle)
Resolution3.85 - 4.8 Å
Structure data

22302

6xsk

EMDB-22302, PDB-6xsk:
Cryo-EM Structure of Vaccine-Elicited Rhesus Antibody 789-203-3C12 in Complex with Stabilized SI06 (A/Solomon Islands/3/06) Influenza Hemagglutinin Trimer
Method: EM (single particle) / Resolution: 3.85 Å

EMDB-26044: H10ssF: ferritin-based nanoparticle displaying H10 hemagglutinin stabilized stem epitopes
Method: EM (single particle) / Resolution: 4.8 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

Source
  • macaca mulatta (Rhesus monkey)
  • influenza a virus (a/solomon islands/3/2006(h1n1))
  • Influenza A virus
  • Helicobacter pylori (bacteria)
KeywordsIMMUNE SYSTEM/Viral Protein / Hemagglutinin ectodomain / Prefusion conformation / Vaccine-elicited antibody / Disulfide-stabilized trimer / IMMUNE SYSTEM / IMMUNE SYSTEM-Viral Protein complex

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