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TitleCryo-EM structure of the needle filament tip complex of the type III secretion injectisome.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 118, Issue 44, Year 2021
Publish dateNov 2, 2021
AuthorsEmily Z Guo / Jorge E Galán /
PubMed AbstractType III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md ...Type III secretion systems are multiprotein molecular machines required for the virulence of several important bacterial pathogens. The central element of these machines is the injectisome, a ∼5-Md multiprotein structure that mediates the delivery of bacterially encoded proteins into eukaryotic target cells. The injectisome is composed of a cytoplasmic sorting platform, and a membrane-embedded needle complex, which is made up of a multiring base and a needle-like filament that extends several nanometers from the bacterial surface. The needle filament is capped at its distal end by another substructure known as the tip complex, which is crucial for the translocation of effector proteins through the eukaryotic cell plasma membrane. Here we report the cryo-EM structure of the Typhimurium needle tip complex docked onto the needle filament tip. Combined with a detailed analysis of structurally guided mutants, this study provides major insight into the assembly and function of this essential component of the type III secretion protein injection machine.
External linksProc Natl Acad Sci U S A / PubMed:34706941 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.7 - 5.0 Å
Structure data

EMDB-24734:
Cryo-EM map of the end of T3SS needle
Method: EM (single particle) / Resolution: 5.0 Å

24735

7rye

EMDB-24735, PDB-7rye:
Cryo-EM structure of the needle filament-tip complex of the Salmonella type III secretion injectisome
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-24736:
Cryo-EM structure of the T3SS needle without tip
Method: EM (helical sym.) / Resolution: 3.8 Å

EMDB-24737:
Cryo-EM map of T3SS needle
Method: EM (helical sym.) / Resolution: 3.7 Å

Source
  • salmonella enterica subsp. enterica serovar typhimurium (bacteria)
KeywordsCELL INVASION / protein secretion / bacterial pathogenesis / organelle assembly

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