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TitleStructure and self-assembly of the calcium binding matrix protein of human metapneumovirus.
Journal, issue, pagesStructure, Vol. 22, Issue 1, Page 136-148, Year 2014
Publish dateJan 7, 2014
AuthorsCedric Leyrat / Max Renner / Karl Harlos / Juha T Huiskonen / Jonathan M Grimes /
PubMed AbstractThe matrix protein (M) of paramyxoviruses plays a key role in determining virion morphology by directing viral assembly and budding. Here, we report the crystal structure of the human metapneumovirus ...The matrix protein (M) of paramyxoviruses plays a key role in determining virion morphology by directing viral assembly and budding. Here, we report the crystal structure of the human metapneumovirus M at 2.8 Å resolution in its native dimeric state. The structure reveals the presence of a high-affinity Ca²⁺ binding site. Molecular dynamics simulations (MDS) predict a secondary lower-affinity site that correlates well with data from fluorescence-based thermal shift assays. By combining small-angle X-ray scattering with MDS and ensemble analysis, we captured the structure and dynamics of M in solution. Our analysis reveals a large positively charged patch on the protein surface that is involved in membrane interaction. Structural analysis of DOPC-induced polymerization of M into helical filaments using electron microscopy leads to a model of M self-assembly. The conservation of the Ca²⁺ binding sites suggests a role for calcium in the replication and morphogenesis of pneumoviruses.
External linksStructure / PubMed:24316400 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution2.83 - 28.0 Å
Structure data

EMDB-2415:
Helical reconstruction of HMPV matrix protein-lipid filaments
Method: EM (helical sym.) / Resolution: 28.0 Å

PDB-4lp7:
Crystal structure of the human metapneumovirus matrix protein
Method: X-RAY DIFFRACTION / Resolution: 2.83 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-CL:
Unknown entry / Chloride

ChemComp-HOH:
WATER / Water

Source
  • human metapneumovirus
  • synthetic construct (others)
KeywordsCALCIUM BINDING PROTEIN / twisted beta sandwich / viral matrix / lipid binding

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