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Structure paper

TitleCryo-EM structure of type 1 IPR channel in a lipid bilayer.
Journal, issue, pagesCommun Biol, Vol. 4, Issue 1, Page 625, Year 2021
Publish dateMay 25, 2021
AuthorsMariah R Baker / Guizhen Fan / Alexander B Seryshev / Melina A Agosto / Matthew L Baker / Irina I Serysheva /
PubMed AbstractType 1 inositol 1,4,5-trisphosphate receptor (IPR1) is the predominant Ca-release channel in neurons. IPR1 mediates Ca release from the endoplasmic reticulum into the cytosol and thereby is involved ...Type 1 inositol 1,4,5-trisphosphate receptor (IPR1) is the predominant Ca-release channel in neurons. IPR1 mediates Ca release from the endoplasmic reticulum into the cytosol and thereby is involved in many physiological processes. Here, we present the cryo-EM structures of full-length rat IPR1 reconstituted in lipid nanodisc and detergent solubilized in the presence of phosphatidylcholine determined in ligand-free, closed states by single-particle electron cryo-microscopy. Notably, both structures exhibit the well-established IPR1 protein fold and reveal a nearly complete representation of lipids with similar locations of ordered lipids bound to the transmembrane domains. The lipid-bound structures show improved features that enabled us to unambiguously build atomic models of IPR1 including two membrane associated helices that were not previously resolved in the TM region. Our findings suggest conserved locations of protein-bound lipids among homotetrameric ion channels that are critical for their structural and functional integrity despite the diversity of structural mechanisms for their gating.
External linksCommun Biol / PubMed:34035440 / PubMed Central
MethodsEM (single particle)
Resolution2.96 - 3.3 Å
Structure data

23337

7lhe

EMDB-23337, PDB-7lhe:
Structure of full-length IP3R1 channel reconstituted into lipid nanodisc in the apo-state
Method: EM (single particle) / Resolution: 3.3 Å

23338

7lhf

EMDB-23338, PDB-7lhf:
Structure of full-length IP3R1 channel solubilized in LNMG & lipid in the apo-state
Method: EM (single particle) / Resolution: 2.96 Å

Chemicals

ChemComp-PLX:
(9R,11S)-9-({[(1S)-1-HYDROXYHEXADECYL]OXY}METHYL)-2,2-DIMETHYL-5,7,10-TRIOXA-2LAMBDA~5~-AZA-6LAMBDA~5~-PHOSPHAOCTACOSANE-6,6,11-TRIOL / phospholipid*YM

ChemComp-ZN:
Unknown entry

Source
  • rattus norvegicus (Norway rat)
KeywordsMEMBRANE PROTEIN / Calcium channel / lipid nanodisc / lipids

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