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Structure paper

TitleThe six steps of the complete F-ATPase rotary catalytic cycle.
Journal, issue, pagesNat Commun, Vol. 12, Issue 1, Page 4690, Year 2021
Publish dateAug 3, 2021
AuthorsMeghna Sobti / Hiroshi Ueno / Hiroyuki Noji / Alastair G Stewart /
PubMed AbstractFF ATP synthase interchanges phosphate transfer energy and proton motive force via a rotary catalysis mechanism. Isolated F-ATPase catalytic cores can hydrolyze ATP, passing through six intermediate ...FF ATP synthase interchanges phosphate transfer energy and proton motive force via a rotary catalysis mechanism. Isolated F-ATPase catalytic cores can hydrolyze ATP, passing through six intermediate conformational states to generate rotation of their central γ-subunit. Although previous structural studies have contributed greatly to understanding rotary catalysis in the F-ATPase, the structure of an important conformational state (the binding-dwell) has remained elusive. Here, we exploit temperature and time-resolved cryo-electron microscopy to determine the structure of the binding- and catalytic-dwell states of Bacillus PS3 F-ATPase. Each state shows three catalytic β-subunits in different conformations, establishing the complete set of six states taken up during the catalytic cycle and providing molecular details for both the ATP binding and hydrolysis strokes. We also identify a potential phosphate-release tunnel that indicates how ADP and phosphate binding are coordinated during synthesis. Overall these findings provide a structural basis for the entire F-ATPase catalytic cycle.
External linksNat Commun / PubMed:34344897 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.6 Å
Structure data

23115

7l1q

EMDB-23115, PDB-7l1q:
PS3 F1-ATPase Binding/TS Dwell
Method: EM (single particle) / Resolution: 3.4 Å

23116

7l1r

EMDB-23116, PDB-7l1r:
PS3 F1-ATPase Hydrolysis Dwell
Method: EM (single particle) / Resolution: 3.1 Å

23117

7l1s

EMDB-23117, PDB-7l1s:
PS3 F1-ATPase Pi-bound Dwell
Method: EM (single particle) / Resolution: 3.6 Å

EMDB-24138:
PS3 F1-ATPase Binding Dwell
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-24139:
PS3 F1-ATPase Catalytic Dwell
Method: EM (single particle) / Resolution: 3.1 Å

EMDB-24140:
PS3 F1-ATPase PiDwell
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-PO4:
PHOSPHATE ION / Phosphate

Source
  • bacillus sp. (strain ps3) (bacteria)
KeywordsTRANSLOCASE / ATPase / ATP synthase

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