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TitleHuman TFIID binds to core promoter DNA in a reorganized structural state.
Journal, issue, pagesCell, Vol. 152, Issue 1-2, Page 120-131, Year 2013
Publish dateJan 17, 2013
AuthorsMichael A Cianfrocco / George A Kassavetis / Patricia Grob / Jie Fang / Tamar Juven-Gershon / James T Kadonaga / Eva Nogales /
PubMed AbstractA mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step ...A mechanistic description of metazoan transcription is essential for understanding the molecular processes that govern cellular decisions. To provide structural insights into the DNA recognition step of transcription initiation, we used single-particle electron microscopy (EM) to visualize human TFIID with promoter DNA. This analysis revealed that TFIID coexists in two predominant and distinct structural states that differ by a 100 Å translocation of TFIID's lobe A. The transition between these structural states is modulated by TFIIA, as the presence of TFIIA and promoter DNA facilitates the formation of a rearranged state of TFIID that enables promoter recognition and binding. DNA labeling and footprinting, together with cryo-EM studies, were used to map the locations of TATA, Initiator (Inr), motif ten element (MTE), and downstream core promoter element (DPE) promoter motifs within the TFIID-TFIIA-DNA structure. The existence of two structurally and functionally distinct forms of TFIID suggests that the different conformers may serve as specific targets for the action of regulatory factors.
External linksCell / PubMed:23332750 / PubMed Central
MethodsEM (single particle)
Resolution32.0 - 35.0 Å
Structure data

EMDB-2282:
TFIIA-induced conformational changes of human TFIID suggest a modular architecture that is required for core promoter specific interactions
Method: EM (single particle) / Resolution: 32.0 Å

EMDB-2283:
Human TFIID Binds to Core Promoter DNA in a Reorganized Structural State
Method: EM (single particle) / Resolution: 32.0 Å

EMDB-2284:
Human TFIID Binds to Core Promoter DNA in a Reorganized Structural State
Method: EM (single particle) / Resolution: 35.0 Å

EMDB-2287:
Human TFIID binds to core promoter DNA in a reorganized structural state
Method: EM (single particle) / Resolution: 35.0 Å

Source
  • Homo sapiens (human)

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