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TitleStructural organization of the C1b projection within the ciliary central apparatus.
Journal, issue, pagesJ Cell Sci, Vol. 134, Issue 21, Year 2021
Publish dateNov 1, 2021
AuthorsKai Cai / Yanhe Zhao / Lei Zhao / Nhan Phan / Yuqing Hou / Xi Cheng / George B Witman / Daniela Nicastro /
PubMed AbstractMotile cilia have a '9+2' structure containing nine doublet microtubules and a central apparatus (CA) composed of two singlet microtubules with associated projections. The CA plays crucial roles in ...Motile cilia have a '9+2' structure containing nine doublet microtubules and a central apparatus (CA) composed of two singlet microtubules with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects in CA assembly or function usually result in motility-impaired or paralyzed cilia, which in humans causes disease. Despite their importance, the protein composition and functions of most CA projections remain largely unknown. Here, we combined genetic, proteomic and cryo-electron tomographic approaches to compare the CA of wild-type Chlamydomonas reinhardtii with those of three CA mutants. Our results show that two proteins, FAP42 and FAP246, are localized to the L-shaped C1b projection of the CA, where they interact with the candidate CA protein FAP413. FAP42 is a large protein that forms the peripheral 'beam' of the C1b projection, and the FAP246-FAP413 subcomplex serves as the 'bracket' between the beam (FAP42) and the C1b 'pillar' that attaches the projection to the C1 microtubule. The FAP246-FAP413-FAP42 complex is essential for stable assembly of the C1b, C1f and C2b projections, and loss of these proteins leads to ciliary motility defects.
External linksJ Cell Sci / PubMed:34651179 / PubMed Central
MethodsEM (subtomogram averaging) / EM (tomography)
Resolution22.0 - 25.0 Å
Structure data

EMDB-22648:
The C1b projection within the ciliary central apparatus from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas wild type axonemes
Method: EM (subtomogram averaging) / Resolution: 23.0 Å

EMDB-22649:
The C1b projection within the the ciliary central apparatus from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap246 axonemes
Method: EM (subtomogram averaging) / Resolution: 22.0 Å

EMDB-22651:
The C1b projection within the the ciliary central apparatus from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap42 axonemes
Method: EM (subtomogram averaging) / Resolution: 25.0 Å

EMDB-24489:
The C1b projection within the ciliary central apparatus from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas fap413 mutant axonemes
Method: EM (tomography) / Resolution: 25.0 Å

Source
  • Chlamydomonas reinhardtii (plant)

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