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TitleCryo-electron microscopy three-dimensional structure of the jumbo phage ΦRSL1 infecting the phytopathogen Ralstonia solanacearum.
Journal, issue, pagesStructure, Vol. 21, Issue 2, Page 298-305, Year 2013
Publish dateFeb 5, 2013
AuthorsGrégory Effantin / Ryosuke Hamasaki / Takeru Kawasaki / Maria Bacia / Christine Moriscot / Winfried Weissenhorn / Takashi Yamada / Guy Schoehn /
PubMed AbstractϕRSL1 jumbo phage belongs to a new class of viruses within the Myoviridae family. Here, we report its three-dimensional structure determined by electron cryo microscopy. The icosahedral capsid, the ...ϕRSL1 jumbo phage belongs to a new class of viruses within the Myoviridae family. Here, we report its three-dimensional structure determined by electron cryo microscopy. The icosahedral capsid, the tail helical portion, and the complete tail appendage were reconstructed separately to resolutions of 9 Å, 9 Å, and 28 Å, respectively. The head is rather complex and formed by at least five different proteins, whereas the major capsid proteins resemble those from HK97, despite low sequence conservation. The helical tail structure demonstrates its close relationship to T4 sheath proteins and provides evidence for an evolutionary link of the inner tail tube to the bacterial type VI secretion apparatus. Long fibers extend from the collar region, and their length is consistent with reaching the host cell surface upon tail contraction. Our structural analyses indicate that ϕRSL1 is an unusual member of the Myoviridae that employs conserved protein machines related to different phages and bacteria.
External linksStructure / PubMed:23394943
MethodsEM (single particle) / EM (helical sym.)
Resolution9.5 - 28.0 Å
Structure data

EMDB-2243:
Cryo-electron microscopy of phirsl1 jumbo phage
Method: EM (single particle) / Resolution: 9.5 Å

EMDB-2244:
Cryo-electron microscopy of phirsl1 jumbo phage
Method: EM (helical sym.) / Resolution: 9.6 Å

EMDB-2245:
Cryo-electron microscopy of phirsl1 jumbo phage
Method: EM (single particle) / Resolution: 28.0 Å

EMDB-2246:
Cryo-electron microscopy of phirsl1 jumbo phage
Method: EM (single particle) / Resolution: 21.0 Å

EMDB-2247:
Cryo-electron microscopy of phirsl1 jumbo phage
Method: EM (single particle) / Resolution: 25.0 Å

Source
  • Ralstonia phage RSL1 (virus)

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